Molecular cloning, characterization and expression analysis of two β-N-acetylhexosaminidase homologs of Coccidioides posadasii

Jennine M. Lunettay, Suzanne M. Johnson, Demosthenes Pappagianis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two full-length cDNAs were isolated from Coccidioides posadasii that encode two deduced proteins (CpHEX1 and CpHEX2) with homology to the glycosyl hydro-lase 20 family of β-N-acetylhexosaminidases. CpHEX1 consists of 595 amino acids, has a predicted molecular mass of 68 kDa and shares the highest identity with the N-acetylhexosaminidase (NAGA) of Aspergillus nidulans, while CpHEX2 consists of 603 amino acids, has a predicted molecular mass of 68.5 kDa and shares the highest identity with NAG1 from Paracoccidioides brasiliensis. CpHEX1 and CpHEX2 share only 23% identity and have dissimilar homologies showing more identity with other fungal β-N-acetylhexosaminidases than with each other. Phylogenetic analysis of selected β-N-acetylhexosaminidases placed CpHEX1 in a cluster with the orthologs from A. nidulans, Aspergillus oryzae, Penicillium chrysogenum and Candida albicans, while CpHEX2 grouped with the orthologs from P. brasiliensis and the Trichoderma spp. β-N- acetylhexosaminidase activity and transcripts encoding CpHEX1 and CpHEX2 were detected in vitro during the spherule-endospore (SE) phase. Expression of the Cphex1 transcript exhibited a temporal increase that correlated with β-N-acetylhexosaminidase activity, while the Cphex2 transcript remained relatively constant. The addition of N-acetylglucosamine to the cultures increased β-N-acetylhexosaminidase activity and the expression of the Cphex1 transcript. A native β-N-acetylhexosaminidase enzyme was purified from in vitro SE phase and identified as CpHEX1 by mass spectrometric analysis. Both the CpHEX1 and CpHEX2 cDNAs were expressed as recombinant fusion proteins and purified under denaturing conditions to apparent homogeneity but they lacked enzymatic activity.

Original languageEnglish (US)
Pages (from-to)744-756
Number of pages13
JournalMedical Mycology
Volume48
Issue number5
DOIs
StatePublished - 2010

Fingerprint

Coccidioides
Paracoccidioides
beta-N-acetylhexosaminidase
Aspergillus nidulans
Molecular Cloning
molecular cloning
Complementary DNA
Penicillium chrysogenum
Recombinant Fusion Proteins
Aspergillus oryzae
Amino Acids
Trichoderma
Acetylglucosamine
Paracoccidioides brasiliensis
Candida albicans
endospores
recombinant fusion proteins
molecular weight
Enzymes
N-acetylglucosamine

Keywords

  • Chitobiase
  • Coccidioides
  • Fungi
  • MRNA expression
  • Purification
  • Rapid amplification of cDNA ends
  • Reverse-transcription polymerase chain reaction
  • β-N- acetylglucosaminidase
  • β-N-acetylhexosaminidase

ASJC Scopus subject areas

  • veterinary(all)
  • Infectious Diseases
  • Medicine(all)

Cite this

Molecular cloning, characterization and expression analysis of two β-N-acetylhexosaminidase homologs of Coccidioides posadasii. / Lunettay, Jennine M.; Johnson, Suzanne M.; Pappagianis, Demosthenes.

In: Medical Mycology, Vol. 48, No. 5, 2010, p. 744-756.

Research output: Contribution to journalArticle

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abstract = "Two full-length cDNAs were isolated from Coccidioides posadasii that encode two deduced proteins (CpHEX1 and CpHEX2) with homology to the glycosyl hydro-lase 20 family of β-N-acetylhexosaminidases. CpHEX1 consists of 595 amino acids, has a predicted molecular mass of 68 kDa and shares the highest identity with the N-acetylhexosaminidase (NAGA) of Aspergillus nidulans, while CpHEX2 consists of 603 amino acids, has a predicted molecular mass of 68.5 kDa and shares the highest identity with NAG1 from Paracoccidioides brasiliensis. CpHEX1 and CpHEX2 share only 23{\%} identity and have dissimilar homologies showing more identity with other fungal β-N-acetylhexosaminidases than with each other. Phylogenetic analysis of selected β-N-acetylhexosaminidases placed CpHEX1 in a cluster with the orthologs from A. nidulans, Aspergillus oryzae, Penicillium chrysogenum and Candida albicans, while CpHEX2 grouped with the orthologs from P. brasiliensis and the Trichoderma spp. β-N- acetylhexosaminidase activity and transcripts encoding CpHEX1 and CpHEX2 were detected in vitro during the spherule-endospore (SE) phase. Expression of the Cphex1 transcript exhibited a temporal increase that correlated with β-N-acetylhexosaminidase activity, while the Cphex2 transcript remained relatively constant. The addition of N-acetylglucosamine to the cultures increased β-N-acetylhexosaminidase activity and the expression of the Cphex1 transcript. A native β-N-acetylhexosaminidase enzyme was purified from in vitro SE phase and identified as CpHEX1 by mass spectrometric analysis. Both the CpHEX1 and CpHEX2 cDNAs were expressed as recombinant fusion proteins and purified under denaturing conditions to apparent homogeneity but they lacked enzymatic activity.",
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AB - Two full-length cDNAs were isolated from Coccidioides posadasii that encode two deduced proteins (CpHEX1 and CpHEX2) with homology to the glycosyl hydro-lase 20 family of β-N-acetylhexosaminidases. CpHEX1 consists of 595 amino acids, has a predicted molecular mass of 68 kDa and shares the highest identity with the N-acetylhexosaminidase (NAGA) of Aspergillus nidulans, while CpHEX2 consists of 603 amino acids, has a predicted molecular mass of 68.5 kDa and shares the highest identity with NAG1 from Paracoccidioides brasiliensis. CpHEX1 and CpHEX2 share only 23% identity and have dissimilar homologies showing more identity with other fungal β-N-acetylhexosaminidases than with each other. Phylogenetic analysis of selected β-N-acetylhexosaminidases placed CpHEX1 in a cluster with the orthologs from A. nidulans, Aspergillus oryzae, Penicillium chrysogenum and Candida albicans, while CpHEX2 grouped with the orthologs from P. brasiliensis and the Trichoderma spp. β-N- acetylhexosaminidase activity and transcripts encoding CpHEX1 and CpHEX2 were detected in vitro during the spherule-endospore (SE) phase. Expression of the Cphex1 transcript exhibited a temporal increase that correlated with β-N-acetylhexosaminidase activity, while the Cphex2 transcript remained relatively constant. The addition of N-acetylglucosamine to the cultures increased β-N-acetylhexosaminidase activity and the expression of the Cphex1 transcript. A native β-N-acetylhexosaminidase enzyme was purified from in vitro SE phase and identified as CpHEX1 by mass spectrometric analysis. Both the CpHEX1 and CpHEX2 cDNAs were expressed as recombinant fusion proteins and purified under denaturing conditions to apparent homogeneity but they lacked enzymatic activity.

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