Molecular cloning and sequencing of the cDNA encoding the feline FcγRIIIA (CD16) homologue

Yorihiro Nishimura, Takayuki Miyazawa, Yasuhiro Ikeda, Yoshihiro Izumiya, Kazuya Nakamura, Eiji Sato, Takeshi Mikami, Eiji Takahashi

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We amplified the cDNA encoding the feline FcγRIIIA (CD16) homologue from peripheral blood mononuclear cells by polymerase chain reaction and cloned two forms of FCGR3A cDNA. Sequencing analysis revealed that the open reading frame of feline FCGR3A cDNA consists of 750 or 747 base pairs encoding 250 or 249 amino acid residues, respectively. Comparison of the predicted amino acid sequence of feline FCGR3A cDNA with those of other mammalians' homologues revealed that the extracellular domain has a relatively low homology. However, the cytoplasmic domain contained an 8-amino acid motif, Leu-Phe-Val-Val-Asp-Thr-Gly-Leu, which was considered to interact with an accessory molecule such as the γ chain of Fc receptors for IgE to form heterodimeric complexes. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)353-359
Number of pages7
JournalVeterinary Immunology and Immunopathology
Volume73
Issue number3-4
DOIs
StatePublished - Mar 15 2000
Externally publishedYes

Keywords

  • Cat
  • CD16
  • cDNA
  • Cloning
  • FcγRIII

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Fingerprint Dive into the research topics of 'Molecular cloning and sequencing of the cDNA encoding the feline FcγRIIIA (CD16) homologue'. Together they form a unique fingerprint.

  • Cite this