Abstract
We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 175-178 |
| Number of pages | 4 |
| Journal | Virus Genes |
| Volume | 18 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1999 |
| Externally published | Yes |
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Keywords
- Lapinized strain
- P gene
- Rinderpest virus
ASJC Scopus subject areas
- Virology
- Molecular Biology
- Immunology
- Applied Microbiology and Biotechnology
- Genetics
Cite this
Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus. / Nakamura, Kazuya; Iwatsuki, Kiyoko; Izumiya, Yoshihiro; Nishimura, Yorihiro; Ohashi, Kenjiro; Mikami, Takeshi; Kai, Chieko.
In: Virus Genes, Vol. 18, No. 2, 1999, p. 175-178.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus
AU - Nakamura, Kazuya
AU - Iwatsuki, Kiyoko
AU - Izumiya, Yoshihiro
AU - Nishimura, Yorihiro
AU - Ohashi, Kenjiro
AU - Mikami, Takeshi
AU - Kai, Chieko
PY - 1999
Y1 - 1999
N2 - We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.
AB - We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.
KW - Lapinized strain
KW - P gene
KW - Rinderpest virus
UR - http://www.scopus.com/inward/record.url?scp=0032972350&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032972350&partnerID=8YFLogxK
U2 - 10.1023/A:1008037222943
DO - 10.1023/A:1008037222943
M3 - Article
C2 - 10403704
AN - SCOPUS:0032972350
VL - 18
SP - 175
EP - 178
JO - Virus Genes
JF - Virus Genes
SN - 0920-8569
IS - 2
ER -