Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus

Kazuya Nakamura, Kiyoko Iwatsuki, Yoshihiro Izumiya, Yorihiro Nishimura, Kenjiro Ohashi, Takeshi Mikami, Chieko Kai

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalVirus Genes
Volume18
Issue number2
DOIs
StatePublished - 1999
Externally publishedYes

Keywords

  • Lapinized strain
  • P gene
  • Rinderpest virus

ASJC Scopus subject areas

  • Virology
  • Molecular Biology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics

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