Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus

Kazuya Nakamura; Kiyoko Iwatsuki; Yoshihiro Izumiya; Yorihiro Nishimura; Kenjiro Ohashi; Takeshi Mikami; Chieko Kai

doi:10.1023/A:1008037222943

Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus

Kazuya Nakamura, Kiyoko Iwatsuki, Yoshihiro Izumiya, Yorihiro Nishimura, Kenjiro Ohashi, Takeshi Mikami, Chieko Kai

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.

Original language	English (US)
Pages (from-to)	175-178
Number of pages	4
Journal	Virus Genes
Volume	18
Issue number	2
DOIs	https://doi.org/10.1023/A:1008037222943
State	Published - 1999
Externally published	Yes

Keywords

Lapinized strain
P gene
Rinderpest virus

ASJC Scopus subject areas

Virology
Molecular Biology
Immunology
Applied Microbiology and Biotechnology
Genetics

Access to Document

10.1023/A:1008037222943

Fingerprint Dive into the research topics of 'Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus'. Together they form a unique fingerprint.

Cite this

@article{435d026b1aea4c52a515f737ef843d3e,

title = "Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus",

abstract = "We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.",

keywords = "Lapinized strain, P gene, Rinderpest virus",

author = "Kazuya Nakamura and Kiyoko Iwatsuki and Yoshihiro Izumiya and Yorihiro Nishimura and Kenjiro Ohashi and Takeshi Mikami and Chieko Kai",

year = "1999",

doi = "10.1023/A:1008037222943",

language = "English (US)",

volume = "18",

pages = "175--178",

journal = "Virus Genes",

issn = "0920-8569",

publisher = "Springer Netherlands",

number = "2",

}

TY - JOUR

T1 - Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus

AU - Nakamura, Kazuya

AU - Iwatsuki, Kiyoko

AU - Izumiya, Yoshihiro

AU - Nishimura, Yorihiro

AU - Ohashi, Kenjiro

AU - Mikami, Takeshi

AU - Kai, Chieko

PY - 1999

Y1 - 1999

N2 - We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.

AB - We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.

KW - Lapinized strain

KW - P gene

KW - Rinderpest virus

UR - http://www.scopus.com/inward/record.url?scp=0032972350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032972350&partnerID=8YFLogxK

U2 - 10.1023/A:1008037222943

DO - 10.1023/A:1008037222943

M3 - Article

C2 - 10403704

AN - SCOPUS:0032972350

VL - 18

SP - 175

EP - 178

JO - Virus Genes

JF - Virus Genes

SN - 0920-8569

IS - 2

ER -