Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus

Kazuya Nakamura, Kiyoko Iwatsuki, Yoshihiro Izumiya, Yorihiro Nishimura, Kenjiro Ohashi, Takeshi Mikami, Chieko Kai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalVirus Genes
Volume18
Issue number2
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Rinderpest virus
Phosphoproteins
Molecular Cloning
Sequence Analysis
Canine Distemper Virus
Measles virus
Genes
Amino Acids
Sequence Alignment
Zinc Fingers
Protein C
Open Reading Frames
Cysteine
Proteins
Nucleotides
Viruses
Messenger RNA

Keywords

  • Lapinized strain
  • P gene
  • Rinderpest virus

ASJC Scopus subject areas

  • Virology
  • Molecular Biology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus. / Nakamura, Kazuya; Iwatsuki, Kiyoko; Izumiya, Yoshihiro; Nishimura, Yorihiro; Ohashi, Kenjiro; Mikami, Takeshi; Kai, Chieko.

In: Virus Genes, Vol. 18, No. 2, 1999, p. 175-178.

Research output: Contribution to journalArticle

Nakamura, K, Iwatsuki, K, Izumiya, Y, Nishimura, Y, Ohashi, K, Mikami, T & Kai, C 1999, 'Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus', Virus Genes, vol. 18, no. 2, pp. 175-178. https://doi.org/10.1023/A:1008037222943
Nakamura, Kazuya ; Iwatsuki, Kiyoko ; Izumiya, Yoshihiro ; Nishimura, Yorihiro ; Ohashi, Kenjiro ; Mikami, Takeshi ; Kai, Chieko. / Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus. In: Virus Genes. 1999 ; Vol. 18, No. 2. pp. 175-178.
@article{435d026b1aea4c52a515f737ef843d3e,
title = "Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus",
abstract = "We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.",
keywords = "Lapinized strain, P gene, Rinderpest virus",
author = "Kazuya Nakamura and Kiyoko Iwatsuki and Yoshihiro Izumiya and Yorihiro Nishimura and Kenjiro Ohashi and Takeshi Mikami and Chieko Kai",
year = "1999",
doi = "10.1023/A:1008037222943",
language = "English (US)",
volume = "18",
pages = "175--178",
journal = "Virus Genes",
issn = "0920-8569",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus

AU - Nakamura, Kazuya

AU - Iwatsuki, Kiyoko

AU - Izumiya, Yoshihiro

AU - Nishimura, Yorihiro

AU - Ohashi, Kenjiro

AU - Mikami, Takeshi

AU - Kai, Chieko

PY - 1999

Y1 - 1999

N2 - We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.

AB - We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV). Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a 'zinc finger' like motif.

KW - Lapinized strain

KW - P gene

KW - Rinderpest virus

UR - http://www.scopus.com/inward/record.url?scp=0032972350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032972350&partnerID=8YFLogxK

U2 - 10.1023/A:1008037222943

DO - 10.1023/A:1008037222943

M3 - Article

C2 - 10403704

AN - SCOPUS:0032972350

VL - 18

SP - 175

EP - 178

JO - Virus Genes

JF - Virus Genes

SN - 0920-8569

IS - 2

ER -