Molecular cloning and expression of feline CD3ε

Yorihiro Nishimura, Takayuki Miyazawa, Yasuhiro Ikeda, Yoshihiro Izumiya, Kazuya Nakamura, Jin Shun Cai, Eiji Sato, Mariko Kohmoto, Takeshi Mikami

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The cDNA of feline CD3ε, one of the T-cell receptor components, was cloned from a feline T-lymphoblastoid cell line (MYA-1 cells) and peripheral blood mononuclear cells and thymocytes of cats by polymerase chain reaction. Sequencing analysis revealed that the open reading frame of feline CD3ε consists of 606 base pairs encoding a predicted molecular mass of 25 kDa transmembrane protein which lacks N-glycosylation site. Comparison of the predicted amino acid sequence of feline CD3ε with those of other mammalians' homologues revealed that a relatively low homology was present in the extracellular domain. However, the cytoplasmic domain contained several characteristic motifs highly conserved across the species. These motifs were known to be important for signal transduction upon T-cell activation or endoplasmic reticulum retention. In addition, the feline CD3ε protein was expressed in an insect cell line (Sf9) by a baculovirus expression system. The expression was confirmed by indirect immunofluorescence assay and immunoblotting analysis using an anti-human CD3ε polyclonal antibody. These results will provide additional information for understanding the feline immune system.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume65
Issue number1
DOIs
StatePublished - Sep 16 1998
Externally publishedYes

Keywords

  • Cat
  • CD3ε
  • cDNA sequence
  • Expression
  • mRNA

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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  • Cite this

    Nishimura, Y., Miyazawa, T., Ikeda, Y., Izumiya, Y., Nakamura, K., Cai, J. S., Sato, E., Kohmoto, M., & Mikami, T. (1998). Molecular cloning and expression of feline CD3ε. Veterinary Immunology and Immunopathology, 65(1), 43-50. https://doi.org/10.1016/S0165-2427(98)00145-7