Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats

Janet E Foley, Shimon Harrus, Amy Poland, Bruno B Chomel, Niels C Pedersen

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152 Citations (Scopus)

Abstract

Objective - To characterize 2 strains of Haemobartonella felis by use of molecular techniques. Animals - 35 specific-pathogen-free cats, 6 months to 4 years old. Procedure - Intraperitoneal or IV inoculation with blood containing H felis small form (Hfsm, 18 cats) or H felis large form (Hflg, 11 cats); 6 cats were uninfected controls. Hfsm was evaluated for capability to cross-protect against the more virulent Hflg. Morphology of both strains was compared by light microscopy of Wright-Giemsa-stained blood smears, and the 16S rRNA genes were sequenced. Results - Infection with Hflg induced signs of depression, fever, and severe macrocytic normochromic anemia with nucleated erythrocytes. More than 95% of erythrocytes were parasitized. Inoculation with Hfsm and uninfected control blood induced mild or no clinical signs and no hematologic abnormalities. Anti-H felis IgG was first detected on postinoculation day (PID) 21, and increased to maximal titer of 400 by PID 28. Reactivated infection was observed in 8 of 29 cats (4 Hfsm and 4 Hflg), with 5% parasitized erythrocytes during the later attack. On PID 8, Hflg-inoculated cats had positive results of polymerase chain reaction analysis (PCR) that persisted until cats were treated with doxycycline or oxytetracycline; Hfsm-inoculated cats had positive PCR results that persisted for duration of observation (3 months). Conclusions - Genetically and morphologically distinct strains of H felis infect cats in the field. The level of genetic difference suggested that these strains may be different species or genera. Clinical Relevance - PCR is a critical diagnostic aid to detect occult Haemobartonella spp infection, as well as response to treatment and clearance of the organism.

Original languageEnglish (US)
Pages (from-to)1581-1588
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume59
Issue number12
StatePublished - Dec 1998

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Mycoplasma haemofelis
Felis
Mycoplasma
Cats
cats
erythrocytes
polymerase chain reaction
Polymerase Chain Reaction
blood
Infection
Erythrocytes
Macrocytic Anemia
infection
Specific Pathogen-Free Organisms
Erythroblasts
Oxytetracycline
doxycycline
Doxycycline
oxytetracycline
rRNA Genes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{305f2cc943e34ad9bc8864b6b1285d94,
title = "Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats",
abstract = "Objective - To characterize 2 strains of Haemobartonella felis by use of molecular techniques. Animals - 35 specific-pathogen-free cats, 6 months to 4 years old. Procedure - Intraperitoneal or IV inoculation with blood containing H felis small form (Hfsm, 18 cats) or H felis large form (Hflg, 11 cats); 6 cats were uninfected controls. Hfsm was evaluated for capability to cross-protect against the more virulent Hflg. Morphology of both strains was compared by light microscopy of Wright-Giemsa-stained blood smears, and the 16S rRNA genes were sequenced. Results - Infection with Hflg induced signs of depression, fever, and severe macrocytic normochromic anemia with nucleated erythrocytes. More than 95{\%} of erythrocytes were parasitized. Inoculation with Hfsm and uninfected control blood induced mild or no clinical signs and no hematologic abnormalities. Anti-H felis IgG was first detected on postinoculation day (PID) 21, and increased to maximal titer of 400 by PID 28. Reactivated infection was observed in 8 of 29 cats (4 Hfsm and 4 Hflg), with 5{\%} parasitized erythrocytes during the later attack. On PID 8, Hflg-inoculated cats had positive results of polymerase chain reaction analysis (PCR) that persisted until cats were treated with doxycycline or oxytetracycline; Hfsm-inoculated cats had positive PCR results that persisted for duration of observation (3 months). Conclusions - Genetically and morphologically distinct strains of H felis infect cats in the field. The level of genetic difference suggested that these strains may be different species or genera. Clinical Relevance - PCR is a critical diagnostic aid to detect occult Haemobartonella spp infection, as well as response to treatment and clearance of the organism.",
author = "Foley, {Janet E} and Shimon Harrus and Amy Poland and Chomel, {Bruno B} and Pedersen, {Niels C}",
year = "1998",
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pages = "1581--1588",
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TY - JOUR

T1 - Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats

AU - Foley, Janet E

AU - Harrus, Shimon

AU - Poland, Amy

AU - Chomel, Bruno B

AU - Pedersen, Niels C

PY - 1998/12

Y1 - 1998/12

N2 - Objective - To characterize 2 strains of Haemobartonella felis by use of molecular techniques. Animals - 35 specific-pathogen-free cats, 6 months to 4 years old. Procedure - Intraperitoneal or IV inoculation with blood containing H felis small form (Hfsm, 18 cats) or H felis large form (Hflg, 11 cats); 6 cats were uninfected controls. Hfsm was evaluated for capability to cross-protect against the more virulent Hflg. Morphology of both strains was compared by light microscopy of Wright-Giemsa-stained blood smears, and the 16S rRNA genes were sequenced. Results - Infection with Hflg induced signs of depression, fever, and severe macrocytic normochromic anemia with nucleated erythrocytes. More than 95% of erythrocytes were parasitized. Inoculation with Hfsm and uninfected control blood induced mild or no clinical signs and no hematologic abnormalities. Anti-H felis IgG was first detected on postinoculation day (PID) 21, and increased to maximal titer of 400 by PID 28. Reactivated infection was observed in 8 of 29 cats (4 Hfsm and 4 Hflg), with 5% parasitized erythrocytes during the later attack. On PID 8, Hflg-inoculated cats had positive results of polymerase chain reaction analysis (PCR) that persisted until cats were treated with doxycycline or oxytetracycline; Hfsm-inoculated cats had positive PCR results that persisted for duration of observation (3 months). Conclusions - Genetically and morphologically distinct strains of H felis infect cats in the field. The level of genetic difference suggested that these strains may be different species or genera. Clinical Relevance - PCR is a critical diagnostic aid to detect occult Haemobartonella spp infection, as well as response to treatment and clearance of the organism.

AB - Objective - To characterize 2 strains of Haemobartonella felis by use of molecular techniques. Animals - 35 specific-pathogen-free cats, 6 months to 4 years old. Procedure - Intraperitoneal or IV inoculation with blood containing H felis small form (Hfsm, 18 cats) or H felis large form (Hflg, 11 cats); 6 cats were uninfected controls. Hfsm was evaluated for capability to cross-protect against the more virulent Hflg. Morphology of both strains was compared by light microscopy of Wright-Giemsa-stained blood smears, and the 16S rRNA genes were sequenced. Results - Infection with Hflg induced signs of depression, fever, and severe macrocytic normochromic anemia with nucleated erythrocytes. More than 95% of erythrocytes were parasitized. Inoculation with Hfsm and uninfected control blood induced mild or no clinical signs and no hematologic abnormalities. Anti-H felis IgG was first detected on postinoculation day (PID) 21, and increased to maximal titer of 400 by PID 28. Reactivated infection was observed in 8 of 29 cats (4 Hfsm and 4 Hflg), with 5% parasitized erythrocytes during the later attack. On PID 8, Hflg-inoculated cats had positive results of polymerase chain reaction analysis (PCR) that persisted until cats were treated with doxycycline or oxytetracycline; Hfsm-inoculated cats had positive PCR results that persisted for duration of observation (3 months). Conclusions - Genetically and morphologically distinct strains of H felis infect cats in the field. The level of genetic difference suggested that these strains may be different species or genera. Clinical Relevance - PCR is a critical diagnostic aid to detect occult Haemobartonella spp infection, as well as response to treatment and clearance of the organism.

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