Molecular characterization of the rat NK cell receptor 2B4

Pappanaicken R. Kumaresan, Susan E. Stepp, Pamela C. Verrett, Samuel S. Chuang, Kent S. Boles, Wayne C. Lai, James C. Ryan, Michael Bennett, Vinay Kumar, Porunelloor A. Mathew

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

2B4 (CD244) is a cell surface glycoprotein of the immunoglobulin superfamily involved in the regulation of natural killer and T lymphocyte function. It is the high affinity counter-receptor for CD48. In mouse and human NK cells, crosslinking of 2B4 with a specific monoclonal antibody or with CD48 can trigger cell-mediated cytotoxicity, IFN-γ secretion, phosphoinositol turnover and NK cell invasiveness. Recent reports of defective 2B4 signaling and NK cell function in X-linked lymphoproliferative syndrome suggest that this may contribute to the progression of this human disease. Here we describe the molecular characterization of the rat 2B4 gene. The cDNA encodes a protein of 395 amino acid residues that contain two Ig domains in the extracellular region and three unique tyrosine motifs (TxYxxV/I/A) in the cytoplasmic region. The predicted protein has 81 and 68% similarity with mouse 2B4 and human 2B4, respectively. Additionally, it has 94 and 89% similarity at the protein level with the recently reported rat 2B4 related genes, r2B4R-tm and r2B4R-se respectively. Northern blot analysis indicated the presence of multiple transcripts in rat LAK cells and RNK-16 cells. Immunoprecipitation and deglycosylation studies showed that rat 2B4 is glycosylated to similar extent as that of mouse and human 2B4. The cloning of r2B4 in the light of the availability of rat NK cell lines should facilitate in vitro and in vivo experiments to decipher the functional role of 2B4 in NK cell biology.

Original languageEnglish (US)
Pages (from-to)735-744
Number of pages10
JournalMolecular Immunology
Volume37
Issue number12-13
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Natural Killer Cell Receptors
Natural Killer Cells
Lymphokine-Activated Killer Cells
B-Cell Antigen Receptors
Proteins
Lymphoproliferative Disorders
Membrane Glycoproteins
Immunoprecipitation
Northern Blotting
Genes
Cell Biology
Tyrosine
Disease Progression
Organism Cloning
Complementary DNA
Monoclonal Antibodies
T-Lymphocytes
Amino Acids
Cell Line

Keywords

  • 2B4
  • CD2 subfamily
  • NK cells
  • Surface receptors

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Kumaresan, P. R., Stepp, S. E., Verrett, P. C., Chuang, S. S., Boles, K. S., Lai, W. C., ... Mathew, P. A. (2001). Molecular characterization of the rat NK cell receptor 2B4. Molecular Immunology, 37(12-13), 735-744. https://doi.org/10.1016/S0161-5890(00)00103-6

Molecular characterization of the rat NK cell receptor 2B4. / Kumaresan, Pappanaicken R.; Stepp, Susan E.; Verrett, Pamela C.; Chuang, Samuel S.; Boles, Kent S.; Lai, Wayne C.; Ryan, James C.; Bennett, Michael; Kumar, Vinay; Mathew, Porunelloor A.

In: Molecular Immunology, Vol. 37, No. 12-13, 2001, p. 735-744.

Research output: Contribution to journalArticle

Kumaresan, PR, Stepp, SE, Verrett, PC, Chuang, SS, Boles, KS, Lai, WC, Ryan, JC, Bennett, M, Kumar, V & Mathew, PA 2001, 'Molecular characterization of the rat NK cell receptor 2B4', Molecular Immunology, vol. 37, no. 12-13, pp. 735-744. https://doi.org/10.1016/S0161-5890(00)00103-6
Kumaresan PR, Stepp SE, Verrett PC, Chuang SS, Boles KS, Lai WC et al. Molecular characterization of the rat NK cell receptor 2B4. Molecular Immunology. 2001;37(12-13):735-744. https://doi.org/10.1016/S0161-5890(00)00103-6
Kumaresan, Pappanaicken R. ; Stepp, Susan E. ; Verrett, Pamela C. ; Chuang, Samuel S. ; Boles, Kent S. ; Lai, Wayne C. ; Ryan, James C. ; Bennett, Michael ; Kumar, Vinay ; Mathew, Porunelloor A. / Molecular characterization of the rat NK cell receptor 2B4. In: Molecular Immunology. 2001 ; Vol. 37, No. 12-13. pp. 735-744.
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AU - Boles, Kent S.

AU - Lai, Wayne C.

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AU - Bennett, Michael

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