Molecular characterization of the Akvr-1 restriction gene

A defective endogenous retrovirus-borne gene identical to Fv-4(r)

Satya Dandekar, P. Rossitto, S. Pickett, G. Mockli, H. Bradshaw, Robert Cardiff, M. Gardner

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A dominant restriction allele, Akvr-1(r), from California wild mice (Mus musculus domesticus) confers resistance to exogenous ecotropic murine leukemia virus (MuLV) infection. The presence of an ecotropic MuLV envelope-related glycoprotein in uninfected virus-resistant cells suggests that viral interference is a possible mechanism for this resistance. We molecularly cloned the ecotropic MuLV envelope-related sequence from the genomic DNA of a wild mouse homozygous for the Akvr-1(r) locus. The cloned provirus was defective and contained a C-terminal end of the pol gene, a complete envelope gene, and a 3' long terminal repeat. The presence of this provirus was directly correlated with Akvr-1(r)-mediated virus resistance in cell cultures and hybrid mice. The Akvr-1(r) provirus restriction map and partial DNA sequence were identical to those of the Fv-4(r) allele, an ecotropic MuLV resistance locus from Japanese feral mice (M. musculus molossinus), which was previously shown to be allelic with the Akvr-1(r) gene. The 3' host flanking sequences of Fv-4(r) and Akvr-1(r) also had identical restriction maps. These findings indicate that Akvr-1(r) and Fv-4(r) are the same gene. It was probably acquired by interbreeding of these feral species in recent times. Conservation of this locus might be favored by the useful function that it performs in protection against ecotropic MuLV infection endemic in both populations of wild mice.

Original languageEnglish (US)
Pages (from-to)308-314
Number of pages7
JournalJournal of Virology
Volume61
Issue number2
StatePublished - 1987

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Retroviridae
Murine leukemia virus
Endogenous Retroviruses
Murine Leukemia Viruses
proviruses
mice
Proviruses
Genes
Mus musculus
genes
loci
Virus Diseases
alleles
viruses
terminal repeat sequences
Viral Interference
Alleles
crossover interference
pol Genes
resistance mechanisms

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular characterization of the Akvr-1 restriction gene : A defective endogenous retrovirus-borne gene identical to Fv-4(r). / Dandekar, Satya; Rossitto, P.; Pickett, S.; Mockli, G.; Bradshaw, H.; Cardiff, Robert; Gardner, M.

In: Journal of Virology, Vol. 61, No. 2, 1987, p. 308-314.

Research output: Contribution to journalArticle

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