Molecular characterization of Sarcocystis neurona strains from opossums (Didelphis virginiana) and intermediate hosts from Central California

Daniel Rejmanek, Melissa A. Miller, Michael E. Grigg, Paul R. Crosbie, Patricia A Conrad

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Sarcocystis neurona is a significant cause of neurological disease in horses and other animals, including the threatened Southern sea otter (Enhydra lutris nereis). Opossums (Didelphis virginiana), the only known definitive hosts for S. neurona in North America, are an introduced species in California. S. neurona DNA isolated from sporocysts and/or infected tissues of 10 opossums, 6 horses, 1 cat, 23 Southern sea otters, and 1 harbor porpoise (Phocoena phocoena) with natural infections was analyzed based on 15 genetic markers, including the first internal transcribed spacer (ITS-1) region; the 25/396 marker; S. neurona surface antigen genes (snSAGs) 2, 3, and 4; and 10 different microsatellites. Based on phylogenetic analysis, most of the S. neurona strains segregated into three genetically distinct groups. Additionally, fifteen S. neurona samples from opossums and several intermediate hosts, including sea otters and horses, were found to be genetically identical across all 15 genetic markers, indicating that fatal encephalitis in Southern sea otters and equine protozoal myeloencephalitis (EPM) in horses is strongly linked to S. neurona sporocysts shed by opossums.

Original languageEnglish (US)
Pages (from-to)20-29
Number of pages10
JournalVeterinary Parasitology
Volume170
Issue number1-2
DOIs
StatePublished - May 2010

Fingerprint

Otters
Didelphis
Sarcocystis
Sarcocystis neurona
Didelphis virginiana
Opossums
opossums
intermediate hosts
Horses
Phocoena
Oocysts
Genetic Markers
Phocoena phocoena
sporocysts (Protozoa)
Horse Diseases
horses
Introduced Species
Encephalomyelitis
Encephalitis
Surface Antigens

Keywords

  • Genetic marker
  • Microsatellite
  • Opossum (Didelphis virginiana)
  • Sarcocystis neurona
  • Sea otter (Enhydra lutris)
  • Surface antigen genes (SAGs)

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Cite this

Molecular characterization of Sarcocystis neurona strains from opossums (Didelphis virginiana) and intermediate hosts from Central California. / Rejmanek, Daniel; Miller, Melissa A.; Grigg, Michael E.; Crosbie, Paul R.; Conrad, Patricia A.

In: Veterinary Parasitology, Vol. 170, No. 1-2, 05.2010, p. 20-29.

Research output: Contribution to journalArticle

Rejmanek, Daniel ; Miller, Melissa A. ; Grigg, Michael E. ; Crosbie, Paul R. ; Conrad, Patricia A. / Molecular characterization of Sarcocystis neurona strains from opossums (Didelphis virginiana) and intermediate hosts from Central California. In: Veterinary Parasitology. 2010 ; Vol. 170, No. 1-2. pp. 20-29.
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