Abstract
Salmonella Paratyphi B dT+ variant (also termed Salmonella Java) and Salmonella Heidelberg are pathogens of public health importance that are frequently isolated from poultry. As a step toward implementing the Colombian Integrated Program for Antimicrobial Resistant Surveillance, this study characterized molecular patterns of Salmonella Paratyphi B dT+ and Salmonella Heidelberg isolated from poultry farms, fecal samples, and retail chicken meat using pulsed-field gel electrophoresis (PFGE). The objective of this study was to determine the genetic relationship among isolates and to determine potential geographically predominant genotypes. Based on PFGE analysis, both serovars exhibited high heterogeneity: the chromosomal DNA fingerprints of 82 Salmonella Paratyphi B dT+ isolates revealed 42 PFGE patterns, whereas the 21 isolates of Salmonella Heidelberg revealed 10 patterns. Similar genotypes of both serovars were demonstrated to be present on farms and in retail outlets. For Salmonella Paratyphi B dT+, closely genetically related strains were found among isolates coming from different farms and different integrated poultry companies within two departments (Santander and Cundinamarca) and also from farms located in the two geographically distant departments. For Salmonella Heidelberg, there were fewer farms with genetically related isolates than for Salmonella Paratyphi B dT+. A possible dissemination of similar genotypes of both serovars along the poultry production chain is hypothesized, and some facilitating factors existing in Colombia are reviewed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 802-807 |
| Number of pages | 6 |
| Journal | Journal of Food Protection |
| Volume | 78 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2015 |
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ASJC Scopus subject areas
- Food Science
- Microbiology
Cite this
Molecular characterization of Salmonella Paratyphi B dT+ and Salmonella Heidelberg from poultry and retail chicken meat in colombia by pulsed-field gel electrophoresis. / Donado-Godoy, Pilar; Byrne, Barbara A; Hume, Michael; León, Maribel; Pérez-Gutiérrez, Enrique; Flores, Martha J Vives; Clavijo, Viviana; Holguin, Ángela; Romero-Zuñiga, Juan J.; Castellanos, Ricardo; Tafur, McAllister; Smith, Woutrina A.
In: Journal of Food Protection, Vol. 78, No. 4, 01.04.2015, p. 802-807.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular characterization of Salmonella Paratyphi B dT+ and Salmonella Heidelberg from poultry and retail chicken meat in colombia by pulsed-field gel electrophoresis
AU - Donado-Godoy, Pilar
AU - Byrne, Barbara A
AU - Hume, Michael
AU - León, Maribel
AU - Pérez-Gutiérrez, Enrique
AU - Flores, Martha J Vives
AU - Clavijo, Viviana
AU - Holguin, Ángela
AU - Romero-Zuñiga, Juan J.
AU - Castellanos, Ricardo
AU - Tafur, McAllister
AU - Smith, Woutrina A
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Salmonella Paratyphi B dT+ variant (also termed Salmonella Java) and Salmonella Heidelberg are pathogens of public health importance that are frequently isolated from poultry. As a step toward implementing the Colombian Integrated Program for Antimicrobial Resistant Surveillance, this study characterized molecular patterns of Salmonella Paratyphi B dT+ and Salmonella Heidelberg isolated from poultry farms, fecal samples, and retail chicken meat using pulsed-field gel electrophoresis (PFGE). The objective of this study was to determine the genetic relationship among isolates and to determine potential geographically predominant genotypes. Based on PFGE analysis, both serovars exhibited high heterogeneity: the chromosomal DNA fingerprints of 82 Salmonella Paratyphi B dT+ isolates revealed 42 PFGE patterns, whereas the 21 isolates of Salmonella Heidelberg revealed 10 patterns. Similar genotypes of both serovars were demonstrated to be present on farms and in retail outlets. For Salmonella Paratyphi B dT+, closely genetically related strains were found among isolates coming from different farms and different integrated poultry companies within two departments (Santander and Cundinamarca) and also from farms located in the two geographically distant departments. For Salmonella Heidelberg, there were fewer farms with genetically related isolates than for Salmonella Paratyphi B dT+. A possible dissemination of similar genotypes of both serovars along the poultry production chain is hypothesized, and some facilitating factors existing in Colombia are reviewed.
AB - Salmonella Paratyphi B dT+ variant (also termed Salmonella Java) and Salmonella Heidelberg are pathogens of public health importance that are frequently isolated from poultry. As a step toward implementing the Colombian Integrated Program for Antimicrobial Resistant Surveillance, this study characterized molecular patterns of Salmonella Paratyphi B dT+ and Salmonella Heidelberg isolated from poultry farms, fecal samples, and retail chicken meat using pulsed-field gel electrophoresis (PFGE). The objective of this study was to determine the genetic relationship among isolates and to determine potential geographically predominant genotypes. Based on PFGE analysis, both serovars exhibited high heterogeneity: the chromosomal DNA fingerprints of 82 Salmonella Paratyphi B dT+ isolates revealed 42 PFGE patterns, whereas the 21 isolates of Salmonella Heidelberg revealed 10 patterns. Similar genotypes of both serovars were demonstrated to be present on farms and in retail outlets. For Salmonella Paratyphi B dT+, closely genetically related strains were found among isolates coming from different farms and different integrated poultry companies within two departments (Santander and Cundinamarca) and also from farms located in the two geographically distant departments. For Salmonella Heidelberg, there were fewer farms with genetically related isolates than for Salmonella Paratyphi B dT+. A possible dissemination of similar genotypes of both serovars along the poultry production chain is hypothesized, and some facilitating factors existing in Colombia are reviewed.
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UR - http://www.scopus.com/inward/citedby.url?scp=84926349936&partnerID=8YFLogxK
U2 - 10.4315/0362-028X.JFP-14-356
DO - 10.4315/0362-028X.JFP-14-356
M3 - Article
C2 - 25836408
AN - SCOPUS:84926349936
VL - 78
SP - 802
EP - 807
JO - Journal of Food Protection
JF - Journal of Food Protection
SN - 0362-028X
IS - 4
ER -