TY - JOUR
T1 - Molecular characterization of newcastle disease viruses isolated from chickens in Tanzania and Ghana
AU - da Silva, Ana P.
AU - Aston, Emily J.
AU - Chiwanga, Gaspar H.
AU - Birakos, Ashley
AU - Muhairwa, Amandus P.
AU - Kayang, Boniface B.
AU - Kelly, Terra
AU - Zhou, Huaijun
AU - Gallardo, Rodrigo A.
PY - 2020/9
Y1 - 2020/9
N2 - Newcastle disease (ND) is one of the most challenging infectious diseases affecting poultry production in Africa, causing major economic losses. To date, Newcastle disease virus isolates from several African countries have been grouped into class II NDV genotypes I, IV, V, VI, VII, XI, XIII, XIV, XVII, XVIII and XXI. Although ND is endemic in many African countries, information on circulating genotypes is still scarce. In Tanzania, outbreaks with genotypes V and XIII have been reported. In West and Central Africa, genotypes XIV, XVII, and XVIII are the most predominant. To investigate other genotypes circulating in Tanzania and Ghana, we performed molecular genotyping on isolates from Tanzania and Ghana using the MinION, a third-generation portable sequencing device from Oxford Nanopore Technologies. Using the MinION, we successfully sequenced the NDV F gene hypervariable region of 24 isolates from Tanzania and four samples from Ghana. In Tanzania, genotypes V, VII and XIII were detected. All isolates from Ghana belonged to genotype XVIII. The data obtained in this study reflect the genetic diversity of NDV in Africa and highlight the importance of surveillance for monitoring the distribution of NDV genotypes and viral evolution.
AB - Newcastle disease (ND) is one of the most challenging infectious diseases affecting poultry production in Africa, causing major economic losses. To date, Newcastle disease virus isolates from several African countries have been grouped into class II NDV genotypes I, IV, V, VI, VII, XI, XIII, XIV, XVII, XVIII and XXI. Although ND is endemic in many African countries, information on circulating genotypes is still scarce. In Tanzania, outbreaks with genotypes V and XIII have been reported. In West and Central Africa, genotypes XIV, XVII, and XVIII are the most predominant. To investigate other genotypes circulating in Tanzania and Ghana, we performed molecular genotyping on isolates from Tanzania and Ghana using the MinION, a third-generation portable sequencing device from Oxford Nanopore Technologies. Using the MinION, we successfully sequenced the NDV F gene hypervariable region of 24 isolates from Tanzania and four samples from Ghana. In Tanzania, genotypes V, VII and XIII were detected. All isolates from Ghana belonged to genotype XVIII. The data obtained in this study reflect the genetic diversity of NDV in Africa and highlight the importance of surveillance for monitoring the distribution of NDV genotypes and viral evolution.
KW - Africa
KW - MinION
KW - Molecular characterization
KW - Nanopore
KW - Newcastle disease
KW - Poultry
UR - http://www.scopus.com/inward/record.url?scp=85089794855&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85089794855&partnerID=8YFLogxK
U2 - 10.3390/v12090916
DO - 10.3390/v12090916
M3 - Article
C2 - 32825492
AN - SCOPUS:85089794855
VL - 12
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 9
M1 - 916
ER -