Molecular characterization of isosporoid coccidia (Isospora and Atoxoplasma spp.) in passerine birds

Mark D. Schrenzel, Gabriel A. Maalouf, Patricia M. Gaffney, Debra Tokarz, Laura L. Keener, Diane McClure, Stephen M Griffey, D. McAloose, Bruce A. Rideout

Research output: Contribution to journalArticle

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Abstract

Prevalence and disease caused by isosporoid coccidia in passerine birds are well recognized, but confusion about the life cycles of the parasites has led to taxonomic inconsistencies. In this study, we characterized segments of the chromosomal small and large-subunit ribosomal RNA (rRNA) genes of coccidial parasites from 23 species of passerine birds, as well as heat shock protein 70, apicoplast rRNA, and chromosomal 5.8s rRNA genes from a subgroup of these animals, and we correlated genetic data with morphologic findings for different parasite developmental stages, host phylogeny, and overall taxonomic relations within the phylum Apicomplexa. Our findings indicate that isosporoid coccidia of passerine birds are monophyletic but exhibit substantial diversity, with most avian species having one or several unique parasite lineages that underwent synchronous speciation with their hosts, interrupted by sporadic episodes of lateral transmission across species and families. Molecular analyses support a homoxenous life cycle, with sexual forms occurring chiefly in the intestines and asexual merozoites present systemically. Rarely, extraintestinal sexual stages can occur. The passerine coccidia are genetically most closely related to species of Eimeria rather than Isospora. We suggest that these parasites, whether identified from blood merozoite stages or fecal oocysts, be provisionally grouped as a homogeneous clade of individual species in a single taxon and formally named when reliable criteria allowing reclassification of related genera in the suborder Eimeriina are clarified.

Original languageEnglish (US)
Pages (from-to)635-647
Number of pages13
JournalJournal of Parasitology
Volume91
Issue number3
DOIs
StatePublished - Jun 2005
Externally publishedYes

Fingerprint

Isospora
Coccidia
passerine
Birds
parasite
Parasites
bird
parasites
birds
Merozoites
merozoites
Life Cycle Stages
RNA
5.8S Ribosomal RNA
life cycle (organisms)
Apicoplasts
life cycle
apicoplasts
Large Ribosome Subunits
ribosomal RNA

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)
  • Ecology, Evolution, Behavior and Systematics

Cite this

Schrenzel, M. D., Maalouf, G. A., Gaffney, P. M., Tokarz, D., Keener, L. L., McClure, D., ... Rideout, B. A. (2005). Molecular characterization of isosporoid coccidia (Isospora and Atoxoplasma spp.) in passerine birds. Journal of Parasitology, 91(3), 635-647. https://doi.org/10.1645/GE-3310

Molecular characterization of isosporoid coccidia (Isospora and Atoxoplasma spp.) in passerine birds. / Schrenzel, Mark D.; Maalouf, Gabriel A.; Gaffney, Patricia M.; Tokarz, Debra; Keener, Laura L.; McClure, Diane; Griffey, Stephen M; McAloose, D.; Rideout, Bruce A.

In: Journal of Parasitology, Vol. 91, No. 3, 06.2005, p. 635-647.

Research output: Contribution to journalArticle

Schrenzel, MD, Maalouf, GA, Gaffney, PM, Tokarz, D, Keener, LL, McClure, D, Griffey, SM, McAloose, D & Rideout, BA 2005, 'Molecular characterization of isosporoid coccidia (Isospora and Atoxoplasma spp.) in passerine birds', Journal of Parasitology, vol. 91, no. 3, pp. 635-647. https://doi.org/10.1645/GE-3310
Schrenzel MD, Maalouf GA, Gaffney PM, Tokarz D, Keener LL, McClure D et al. Molecular characterization of isosporoid coccidia (Isospora and Atoxoplasma spp.) in passerine birds. Journal of Parasitology. 2005 Jun;91(3):635-647. https://doi.org/10.1645/GE-3310
Schrenzel, Mark D. ; Maalouf, Gabriel A. ; Gaffney, Patricia M. ; Tokarz, Debra ; Keener, Laura L. ; McClure, Diane ; Griffey, Stephen M ; McAloose, D. ; Rideout, Bruce A. / Molecular characterization of isosporoid coccidia (Isospora and Atoxoplasma spp.) in passerine birds. In: Journal of Parasitology. 2005 ; Vol. 91, No. 3. pp. 635-647.
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