TY - JOUR
T1 - Molecular characterization of human immunodeficiency virus from Zaire
T2 - nucleotide sequence analysis identifies conserved and variable domains in the envelope gene
AU - Srinivasan, A.
AU - Anand, R.
AU - York, D.
AU - Ranganathan, P.
AU - Feorino, P.
AU - Schochetman, G.
AU - Curran, J.
AU - Kalyanaraman, V. S.
AU - Luciw, Paul A
AU - Sanchez-Pescador, R.
PY - 1987
Y1 - 1987
N2 - To examine the genetic relatedness of human immunodeficiency viruses (HIV) from different geographic locations, we molecularly cloned the genome of HIV isolated from a Zairian AIDS patient. Restriction mapping of the recombinant clone, designated HIV-Zr6, revealed both common (as observed in other HIV isolates) and unique restriction sites. The DNA clone of HIV-Zr6, shown to give rise to infectious cytopathic virus after transfection of cultured lymphoid cells, was sequenced in several regions. The long terminal repeat (LTR), open reading frame 1 (ORF1), C-terminal envelope (env) gene domain, and ORF2 showed less than 6% difference in nucleotide sequence when compared to other HIV isolates including human T-lymphotropic virus-type III (HTLV-III) clone BIO, lymphadenopathy-associated virus-1 (LAV-1), and AIDS-associated retrovirus-2 (ARV-2). About 15% difference in nucleotide sequences was noted in the N-terminal env gene domain. Alignments of en v gene sequences revealed conserved, moderately variable, and hypervariable stretches in the predicted amino acid sequences. This model provides a basis for assessing the significance of sequence variation on properties controlled by the viral Env glycoproteins such as cell tropism and immunogenicity.
AB - To examine the genetic relatedness of human immunodeficiency viruses (HIV) from different geographic locations, we molecularly cloned the genome of HIV isolated from a Zairian AIDS patient. Restriction mapping of the recombinant clone, designated HIV-Zr6, revealed both common (as observed in other HIV isolates) and unique restriction sites. The DNA clone of HIV-Zr6, shown to give rise to infectious cytopathic virus after transfection of cultured lymphoid cells, was sequenced in several regions. The long terminal repeat (LTR), open reading frame 1 (ORF1), C-terminal envelope (env) gene domain, and ORF2 showed less than 6% difference in nucleotide sequence when compared to other HIV isolates including human T-lymphotropic virus-type III (HTLV-III) clone BIO, lymphadenopathy-associated virus-1 (LAV-1), and AIDS-associated retrovirus-2 (ARV-2). About 15% difference in nucleotide sequences was noted in the N-terminal env gene domain. Alignments of en v gene sequences revealed conserved, moderately variable, and hypervariable stretches in the predicted amino acid sequences. This model provides a basis for assessing the significance of sequence variation on properties controlled by the viral Env glycoproteins such as cell tropism and immunogenicity.
KW - African AIDS
KW - cloning
KW - lymphoid cells
KW - Recombinant DNA
KW - restriction mapping
KW - λ phage vectors
UR - http://www.scopus.com/inward/record.url?scp=0023198758&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023198758&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(87)90396-9
DO - 10.1016/0378-1119(87)90396-9
M3 - Article
C2 - 3036660
AN - SCOPUS:0023198758
VL - 52
SP - 71
EP - 82
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -