Molecular characterization of human immunodeficiency virus from Zaire: nucleotide sequence analysis identifies conserved and variable domains in the envelope gene

A. Srinivasan, R. Anand, D. York, P. Ranganathan, P. Feorino, G. Schochetman, J. Curran, V. S. Kalyanaraman, Paul A Luciw, R. Sanchez-Pescador

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

To examine the genetic relatedness of human immunodeficiency viruses (HIV) from different geographic locations, we molecularly cloned the genome of HIV isolated from a Zairian AIDS patient. Restriction mapping of the recombinant clone, designated HIV-Zr6, revealed both common (as observed in other HIV isolates) and unique restriction sites. The DNA clone of HIV-Zr6, shown to give rise to infectious cytopathic virus after transfection of cultured lymphoid cells, was sequenced in several regions. The long terminal repeat (LTR), open reading frame 1 (ORF1), C-terminal envelope (env) gene domain, and ORF2 showed less than 6% difference in nucleotide sequence when compared to other HIV isolates including human T-lymphotropic virus-type III (HTLV-III) clone BIO, lymphadenopathy-associated virus-1 (LAV-1), and AIDS-associated retrovirus-2 (ARV-2). About 15% difference in nucleotide sequences was noted in the N-terminal env gene domain. Alignments of en v gene sequences revealed conserved, moderately variable, and hypervariable stretches in the predicted amino acid sequences. This model provides a basis for assessing the significance of sequence variation on properties controlled by the viral Env glycoproteins such as cell tropism and immunogenicity.

Original languageEnglish (US)
Pages (from-to)71-82
Number of pages12
JournalGene
Volume52
Issue number1
DOIs
StatePublished - 1987

    Fingerprint

Keywords

  • African AIDS
  • cloning
  • lymphoid cells
  • Recombinant DNA
  • restriction mapping
  • λ phage vectors

ASJC Scopus subject areas

  • Genetics

Cite this

Srinivasan, A., Anand, R., York, D., Ranganathan, P., Feorino, P., Schochetman, G., Curran, J., Kalyanaraman, V. S., Luciw, P. A., & Sanchez-Pescador, R. (1987). Molecular characterization of human immunodeficiency virus from Zaire: nucleotide sequence analysis identifies conserved and variable domains in the envelope gene. Gene, 52(1), 71-82. https://doi.org/10.1016/0378-1119(87)90396-9