Molecular characterization of first human Bartonella strain isolated in Italy

A. Ciervo, A. Petrucca, S. Ciarrocchi, A. Pinto, L. Bonazzi, A. Fabio, E. Farnetti, Bruno B Chomel, L. Ciceroni

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Abstract

The aim of this study was to characterize a Bartonella strain (BA-1) isolated from a blood culture of an Italian, human immunodeficiency virus-positive patient with bacillary angiomatosis. We analyzed the isolate using molecular biology methods such as whole-cell fatty acid analysis, PCR-restriction fragment length polymorphism analysis, type-specific 16S rRNA PCRs, sequence analysis of the 16S rRNA, pulsed-field gel electrophoresis, and arbitrarily primed PCR. The BA-1 isolate turned out to be a Bartonella quintana strain, similar but not identical to B. quintana Oldahoma, which was used as a control strain.

Original languageEnglish (US)
Pages (from-to)4554-4557
Number of pages4
JournalJournal of Clinical Microbiology
Volume39
Issue number12
DOIs
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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    Ciervo, A., Petrucca, A., Ciarrocchi, S., Pinto, A., Bonazzi, L., Fabio, A., Farnetti, E., Chomel, B. B., & Ciceroni, L. (2001). Molecular characterization of first human Bartonella strain isolated in Italy. Journal of Clinical Microbiology, 39(12), 4554-4557. https://doi.org/10.1128/JCM.39.12.4554-4557.2001