Abstract
Interleukin 16 (IL-16) has been shown to diminish HIV and SIV replication through inhibition of HIV and SIV mRNA transcription. To evaluate its role in the FIV cat model, we cloned and expressed feline IL-16 and determined its ability to induce chemotaxis as well as to inhibit FIV replication in cultured PBMCs. Sequence comparison of rfIL-16 with human, African green monkey, rhesus macaque, and mouse IL-16 showed 84.2, 84.5, 84.4, and 79.4% identity at the nucleotide sequence level and 93, 91.5, 90.7, and 87.2% identity at the amino acid sequence level, respectively. Biocharacterization of rfIL,16 revealed potent induction of chemotaxis (p < 0.05). In addition, p24 production from feline PBMCS infected with FIV Zurich 2 in vitro was decreased up to 87% (p < 0.05). These data demonstrate biologic and antiviral functionality of rfIL-16.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 569-575 |
| Number of pages | 7 |
| Journal | AIDS Research and Human Retroviruses |
| Volume | 16 |
| Issue number | 6 |
| DOIs | |
| State | Published - Apr 10 2000 |
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ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Molecular characterization of feline interleukin 16 : Chemotactic activity and effect on feline immunodeficiency virus infection and/or replication. / Leutenegger, Christian M.; huder, Jon B.; Mislin, Caroline N.; Lahrtz, Fritz; Hofmann-Lehmann, Regina; Pedersen, Niels C; Lutz, Hans.
In: AIDS Research and Human Retroviruses, Vol. 16, No. 6, 10.04.2000, p. 569-575.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular characterization of feline interleukin 16
T2 - Chemotactic activity and effect on feline immunodeficiency virus infection and/or replication
AU - Leutenegger, Christian M.
AU - huder, Jon B.
AU - Mislin, Caroline N.
AU - Lahrtz, Fritz
AU - Hofmann-Lehmann, Regina
AU - Pedersen, Niels C
AU - Lutz, Hans
PY - 2000/4/10
Y1 - 2000/4/10
N2 - Interleukin 16 (IL-16) has been shown to diminish HIV and SIV replication through inhibition of HIV and SIV mRNA transcription. To evaluate its role in the FIV cat model, we cloned and expressed feline IL-16 and determined its ability to induce chemotaxis as well as to inhibit FIV replication in cultured PBMCs. Sequence comparison of rfIL-16 with human, African green monkey, rhesus macaque, and mouse IL-16 showed 84.2, 84.5, 84.4, and 79.4% identity at the nucleotide sequence level and 93, 91.5, 90.7, and 87.2% identity at the amino acid sequence level, respectively. Biocharacterization of rfIL,16 revealed potent induction of chemotaxis (p < 0.05). In addition, p24 production from feline PBMCS infected with FIV Zurich 2 in vitro was decreased up to 87% (p < 0.05). These data demonstrate biologic and antiviral functionality of rfIL-16.
AB - Interleukin 16 (IL-16) has been shown to diminish HIV and SIV replication through inhibition of HIV and SIV mRNA transcription. To evaluate its role in the FIV cat model, we cloned and expressed feline IL-16 and determined its ability to induce chemotaxis as well as to inhibit FIV replication in cultured PBMCs. Sequence comparison of rfIL-16 with human, African green monkey, rhesus macaque, and mouse IL-16 showed 84.2, 84.5, 84.4, and 79.4% identity at the nucleotide sequence level and 93, 91.5, 90.7, and 87.2% identity at the amino acid sequence level, respectively. Biocharacterization of rfIL,16 revealed potent induction of chemotaxis (p < 0.05). In addition, p24 production from feline PBMCS infected with FIV Zurich 2 in vitro was decreased up to 87% (p < 0.05). These data demonstrate biologic and antiviral functionality of rfIL-16.
UR - http://www.scopus.com/inward/record.url?scp=0034630301&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034630301&partnerID=8YFLogxK
U2 - 10.1089/088922200308981
DO - 10.1089/088922200308981
M3 - Article
C2 - 10777147
AN - SCOPUS:0034630301
VL - 16
SP - 569
EP - 575
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
SN - 0889-2229
IS - 6
ER -