Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato

Steven R. Scofield; Christian M. Tobias; John P. Rathjen; Jeff H. Chang; Daniel T. Lavelle; Richard W Michelmore; Brian J. Staskawicz

doi:10.1126/science.274.5295.2063

Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato

Steven R. Scofield, Christian M. Tobias, John P. Rathjen, Jeff H. Chang, Daniel T. Lavelle, Richard W Michelmore, Brian J. Staskawicz

Medical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

431 Scopus citations

Abstract

Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two- hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.

Original language	English (US)
Pages (from-to)	2063-2065
Number of pages	3
Journal	Science
Volume	274
Issue number	5295
DOIs	https://doi.org/10.1126/science.274.5295.2063
State	Published - Dec 20 1996

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title = "Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato",

abstract = "Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two- hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.",

author = "Scofield, {Steven R.} and Tobias, {Christian M.} and Rathjen, {John P.} and Chang, {Jeff H.} and Lavelle, {Daniel T.} and Michelmore, {Richard W} and Staskawicz, {Brian J.}",

year = "1996",

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doi = "10.1126/science.274.5295.2063",

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T1 - Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato

AU - Scofield, Steven R.

AU - Tobias, Christian M.

AU - Rathjen, John P.

AU - Chang, Jeff H.

AU - Lavelle, Daniel T.

AU - Michelmore, Richard W

AU - Staskawicz, Brian J.

PY - 1996/12/20

Y1 - 1996/12/20

N2 - Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two- hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.

AB - Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two- hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.

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Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato

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