Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato

Steven R. Scofield, Christian M. Tobias, John P. Rathjen, Jeff H. Chang, Daniel T. Lavelle, Richard W Michelmore, Brian J. Staskawicz

Research output: Contribution to journal › Article

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Abstract

Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two- hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.

Original languageEnglish (US)
Pages (from-to)2063-2065
Number of pages3
JournalScience
Volume274
Issue number5295
DOIs
StatePublished - Dec 20 1996

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two hybrid system techniques
tomatoes
Pseudomonas syringae
necrosis
genes
host plants
proteins
cells
yeasts
mutation
pathogens
infection

ASJC Scopus subject areas

  • General

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Scofield, S. R., Tobias, C. M., Rathjen, J. P., Chang, J. H., Lavelle, D. T., Michelmore, R. W., & Staskawicz, B. J. (1996). Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato. Science, 274(5295), 2063-2065. https://doi.org/10.1126/science.274.5295.2063

Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato. / Scofield, Steven R.; Tobias, Christian M.; Rathjen, John P.; Chang, Jeff H.; Lavelle, Daniel T.; Michelmore, Richard W; Staskawicz, Brian J.

In: Science, Vol. 274, No. 5295, 20.12.1996, p. 2063-2065.

Research output: Contribution to journal › Article

Scofield, SR, Tobias, CM, Rathjen, JP, Chang, JH, Lavelle, DT, Michelmore, RW & Staskawicz, BJ 1996, 'Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato', Science, vol. 274, no. 5295, pp. 2063-2065. https://doi.org/10.1126/science.274.5295.2063
Scofield SR, Tobias CM, Rathjen JP, Chang JH, Lavelle DT, Michelmore RW et al. Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato. Science. 1996 Dec 20;274(5295):2063-2065. https://doi.org/10.1126/science.274.5295.2063
Scofield, Steven R. ; Tobias, Christian M. ; Rathjen, John P. ; Chang, Jeff H. ; Lavelle, Daniel T. ; Michelmore, Richard W ; Staskawicz, Brian J. / Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato. In: Science. 1996 ; Vol. 274, No. 5295. pp. 2063-2065.
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