Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato

Steven R. Scofield, Christian M. Tobias, John P. Rathjen, Jeff H. Chang, Daniel T. Lavelle, Richard W Michelmore, Brian J. Staskawicz

Research output: Contribution to journalArticle

418 Citations (Scopus)

Abstract

Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two- hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.

Original languageEnglish (US)
Pages (from-to)2063-2065
Number of pages3
JournalScience
Volume274
Issue number5295
DOIs
StatePublished - Dec 20 1996

Fingerprint

two hybrid system techniques
tomatoes
Pseudomonas syringae
necrosis
genes
host plants
proteins
cells
yeasts
mutation
pathogens
infection

ASJC Scopus subject areas

  • General

Cite this

Scofield, S. R., Tobias, C. M., Rathjen, J. P., Chang, J. H., Lavelle, D. T., Michelmore, R. W., & Staskawicz, B. J. (1996). Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato. Science, 274(5295), 2063-2065. https://doi.org/10.1126/science.274.5295.2063

Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato. / Scofield, Steven R.; Tobias, Christian M.; Rathjen, John P.; Chang, Jeff H.; Lavelle, Daniel T.; Michelmore, Richard W; Staskawicz, Brian J.

In: Science, Vol. 274, No. 5295, 20.12.1996, p. 2063-2065.

Research output: Contribution to journalArticle

Scofield, SR, Tobias, CM, Rathjen, JP, Chang, JH, Lavelle, DT, Michelmore, RW & Staskawicz, BJ 1996, 'Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato', Science, vol. 274, no. 5295, pp. 2063-2065. https://doi.org/10.1126/science.274.5295.2063
Scofield SR, Tobias CM, Rathjen JP, Chang JH, Lavelle DT, Michelmore RW et al. Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato. Science. 1996 Dec 20;274(5295):2063-2065. https://doi.org/10.1126/science.274.5295.2063
Scofield, Steven R. ; Tobias, Christian M. ; Rathjen, John P. ; Chang, Jeff H. ; Lavelle, Daniel T. ; Michelmore, Richard W ; Staskawicz, Brian J. / Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato. In: Science. 1996 ; Vol. 274, No. 5295. pp. 2063-2065.
@article{6249606eff5149508c2e4374e3a3272b,
title = "Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato",
abstract = "Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two- hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.",
author = "Scofield, {Steven R.} and Tobias, {Christian M.} and Rathjen, {John P.} and Chang, {Jeff H.} and Lavelle, {Daniel T.} and Michelmore, {Richard W} and Staskawicz, {Brian J.}",
year = "1996",
month = "12",
day = "20",
doi = "10.1126/science.274.5295.2063",
language = "English (US)",
volume = "274",
pages = "2063--2065",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5295",

}

TY - JOUR

T1 - Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato

AU - Scofield, Steven R.

AU - Tobias, Christian M.

AU - Rathjen, John P.

AU - Chang, Jeff H.

AU - Lavelle, Daniel T.

AU - Michelmore, Richard W

AU - Staskawicz, Brian J.

PY - 1996/12/20

Y1 - 1996/12/20

N2 - Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two- hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.

AB - Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two- hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.

UR - http://www.scopus.com/inward/record.url?scp=0030475465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030475465&partnerID=8YFLogxK

U2 - 10.1126/science.274.5295.2063

DO - 10.1126/science.274.5295.2063

M3 - Article

AN - SCOPUS:0030475465

VL - 274

SP - 2063

EP - 2065

JO - Science

JF - Science

SN - 0036-8075

IS - 5295

ER -