Molecular and immunohistochemical diagnosis of Francisella noatunensis subsp. orientalis from formalin-fixed, paraffin-embedded tissues

Esteban Soto Martinez, Oscar Illanes, David Hilchie, Juan A. Morales, Piyanate Sunyakumthorn, John P. Hawke, Andrew E. Goodwin, Allen Riggs, Roy P. Yanong, Deborah B. Pouder, Ruth Francis-Floyd, Maziel Arauz, Lewis Bogdanovic, Fernanda Castillo-Alcala

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Members of the genus Francisella (viz., F. noatunensis subsp. orientalis [Fno] and F. noatunensis subsp. noatunensis) have been described as causative agents of chronic granulomatous and pyogranulomatous lesions in wild and cultured fish species. In the present study, 68 archived formalin-fixed, paraffin-embedded (FFPE) tissues from several fish species, collected at different geographical locations from 2000 to 2011, were analyzed using a real-time polymerase chain reaction assay for the detection of the Fno intracellular growth loci C (iglC) gene and by immunohistochemistry for the demonstration of Fno antigens. The results revealed a high correlation between these 2 diagnostic techniques validating their use for the diagnosis of Fno infection in archived FFPE tissues and confirming the presence of Fno in fish species from the Cari y years of the present century.

Original languageEnglish (US)
Pages (from-to)840-845
Number of pages6
JournalJournal of Veterinary Diagnostic Investigation
Volume24
Issue number5
DOIs
StatePublished - Sep 2012
Externally publishedYes

Keywords

  • Francisella noatunensis
  • iglC
  • immunohistochemistry
  • real-time polymerase chain reaction

ASJC Scopus subject areas

  • veterinary(all)

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