Molecular and immunohistochemical diagnosis of Francisella noatunensis subsp. orientalis from formalin-fixed, paraffin-embedded tissues

Esteban Soto Martinez, Oscar Illanes, David Hilchie, Juan A. Morales, Piyanate Sunyakumthorn, John P. Hawke, Andrew E. Goodwin, Allen Riggs, Roy P. Yanong, Deborah B. Pouder, Ruth Francis-Floyd, Maziel Arauz, Lewis Bogdanovic, Fernanda Castillo-Alcala

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Members of the genus Francisella (viz., F. noatunensis subsp. orientalis [Fno] and F. noatunensis subsp. noatunensis) have been described as causative agents of chronic granulomatous and pyogranulomatous lesions in wild and cultured fish species. In the present study, 68 archived formalin-fixed, paraffin-embedded (FFPE) tissues from several fish species, collected at different geographical locations from 2000 to 2011, were analyzed using a real-time polymerase chain reaction assay for the detection of the Fno intracellular growth loci C (iglC) gene and by immunohistochemistry for the demonstration of Fno antigens. The results revealed a high correlation between these 2 diagnostic techniques validating their use for the diagnosis of Fno infection in archived FFPE tissues and confirming the presence of Fno in fish species from the Cari y years of the present century.

Original languageEnglish (US)
Pages (from-to)840-845
Number of pages6
JournalJournal of Veterinary Diagnostic Investigation
Volume24
Issue number5
DOIs
StatePublished - Sep 2012
Externally publishedYes

Fingerprint

Francisella
formalin
Paraffin
alkanes
Formaldehyde
Fishes
fish
Geographical Locations
lesions (animal)
diagnostic techniques
immunohistochemistry
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Immunohistochemistry
antigens
Antigens
loci
assays
Growth
Infection

Keywords

  • Francisella noatunensis
  • iglC
  • immunohistochemistry
  • real-time polymerase chain reaction

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Molecular and immunohistochemical diagnosis of Francisella noatunensis subsp. orientalis from formalin-fixed, paraffin-embedded tissues. / Soto Martinez, Esteban; Illanes, Oscar; Hilchie, David; Morales, Juan A.; Sunyakumthorn, Piyanate; Hawke, John P.; Goodwin, Andrew E.; Riggs, Allen; Yanong, Roy P.; Pouder, Deborah B.; Francis-Floyd, Ruth; Arauz, Maziel; Bogdanovic, Lewis; Castillo-Alcala, Fernanda.

In: Journal of Veterinary Diagnostic Investigation, Vol. 24, No. 5, 09.2012, p. 840-845.

Research output: Contribution to journalArticle

Soto Martinez, E, Illanes, O, Hilchie, D, Morales, JA, Sunyakumthorn, P, Hawke, JP, Goodwin, AE, Riggs, A, Yanong, RP, Pouder, DB, Francis-Floyd, R, Arauz, M, Bogdanovic, L & Castillo-Alcala, F 2012, 'Molecular and immunohistochemical diagnosis of Francisella noatunensis subsp. orientalis from formalin-fixed, paraffin-embedded tissues', Journal of Veterinary Diagnostic Investigation, vol. 24, no. 5, pp. 840-845. https://doi.org/10.1177/1040638712452108
Soto Martinez, Esteban ; Illanes, Oscar ; Hilchie, David ; Morales, Juan A. ; Sunyakumthorn, Piyanate ; Hawke, John P. ; Goodwin, Andrew E. ; Riggs, Allen ; Yanong, Roy P. ; Pouder, Deborah B. ; Francis-Floyd, Ruth ; Arauz, Maziel ; Bogdanovic, Lewis ; Castillo-Alcala, Fernanda. / Molecular and immunohistochemical diagnosis of Francisella noatunensis subsp. orientalis from formalin-fixed, paraffin-embedded tissues. In: Journal of Veterinary Diagnostic Investigation. 2012 ; Vol. 24, No. 5. pp. 840-845.
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