Molecular and functional characterization of carp TNF: A link between TNF polymorphism and trypanotolerance?

Jeroen Saeij, René J M Stet, Beja J. De Vries, Willem B. Van Muiswinkel, Geert F. Wiegertjes

Research output: Contribution to journalArticle

128 Scopus citations


Two carp tumor necrosis factor alpha (TNFα) genes have been cloned and sequenced. Both TNF1 and TNF2 sequences have several polymorphisms in the 3′ UTR and TNF2 has a polymorphism in the coding sequence. Lipopolysaccharide and the protozoan blood flagellate Trypanoplasma borreli induced expression of TNFα in carp head kidney phagocytes when added in vitro. Differential expression was observed, with TNF2 being higher expressed than TNF1. We used the TNFα-specific inhibitor pentoxifylline to demonstrate the involvement of carp TNFα in the induction of nitric oxide and in the stimulation of cell proliferation. In addition, two carp lines differing in their resistance to T. borreli were typed for the TNF2 polymorphism and association between one isoform and resistance was found.

Original languageEnglish (US)
Pages (from-to)29-41
Number of pages13
JournalDevelopmental and Comparative Immunology
Issue number1
StatePublished - Jan 2003
Externally publishedYes



  • Cytokine
  • Fish
  • Infection
  • Kinetoplastida
  • Nitric oxide
  • Parasite
  • Phagocyte
  • TNF

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

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