Molecular and functional characterization of carp TNF

A link between TNF polymorphism and trypanotolerance?

Jeroen Saeij, René J M Stet, Beja J. De Vries, Willem B. Van Muiswinkel, Geert F. Wiegertjes

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Two carp tumor necrosis factor alpha (TNFα) genes have been cloned and sequenced. Both TNF1 and TNF2 sequences have several polymorphisms in the 3′ UTR and TNF2 has a polymorphism in the coding sequence. Lipopolysaccharide and the protozoan blood flagellate Trypanoplasma borreli induced expression of TNFα in carp head kidney phagocytes when added in vitro. Differential expression was observed, with TNF2 being higher expressed than TNF1. We used the TNFα-specific inhibitor pentoxifylline to demonstrate the involvement of carp TNFα in the induction of nitric oxide and in the stimulation of cell proliferation. In addition, two carp lines differing in their resistance to T. borreli were typed for the TNF2 polymorphism and association between one isoform and resistance was found.

Original languageEnglish (US)
Pages (from-to)29-41
Number of pages13
JournalDevelopmental and Comparative Immunology
Volume27
Issue number1
DOIs
StatePublished - Jan 2003
Externally publishedYes

Fingerprint

Carps
Tumor Necrosis Factor-alpha
Head Kidney
Pentoxifylline
3' Untranslated Regions
Phagocytes
Lipopolysaccharides
Nitric Oxide
Protein Isoforms
Cell Proliferation
Genes

Keywords

  • Cytokine
  • Fish
  • Infection
  • Kinetoplastida
  • Nitric oxide
  • Parasite
  • Phagocyte
  • TNF

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Molecular and functional characterization of carp TNF : A link between TNF polymorphism and trypanotolerance? / Saeij, Jeroen; Stet, René J M; De Vries, Beja J.; Van Muiswinkel, Willem B.; Wiegertjes, Geert F.

In: Developmental and Comparative Immunology, Vol. 27, No. 1, 01.2003, p. 29-41.

Research output: Contribution to journalArticle

Saeij, Jeroen ; Stet, René J M ; De Vries, Beja J. ; Van Muiswinkel, Willem B. ; Wiegertjes, Geert F. / Molecular and functional characterization of carp TNF : A link between TNF polymorphism and trypanotolerance?. In: Developmental and Comparative Immunology. 2003 ; Vol. 27, No. 1. pp. 29-41.
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