Molecular analysis of isolates of Streptoccocus suis capsular type 2 by restriction-endonuclease-digested DNA separated on SDS-PAGE and by hybridization with an rDNA probe

M. Beaudoin, J. Harel, Robert Higgins, M. Gottschalk, M. Frenette, J. I. MacInnes

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This study was undertaken to assess the discriminatory value of restriction endonuclease (RE) digestion patterns of Streptococcus suis chromosomal DNA using polyacrylamide gel electrophoresis (SDS-PAGE) and DNA-rDNA hybridization. For the RE digestion patterns, DNAs were digested separately with the enzymes BamHI and BglII and the resultant fragments were separated by SDS-PAGE. An Escherichia coli rDNA probe derived from pKK3535 was used for the hybridization. Twenty-three S. suis capsular type 2 isolates recovered from diseased and clinically healthy pigs, from a human case, and from a cow were compared in this study. The majority of isolates associated with septicaemia belonged to one restriction endonuclease analysis (REA) profile group. Isolates associated with pneumonia belonged either to the REA profile group of isolates associated with septicaemia or to a second REA profile group. The REA profiles of isolates from clinically healthy animals were more heterogeneous. The REA profile of the type 2 reference strain, S735, which was originally isolated from a pig, was very different from those of the porcine and bovine isolates but similar to the profile of the human isolate. The profiles obtained after rDNA hybridization were more homogeneous. Although different patterns were detected in the 23 isolates, there was no correlation between the source of the isolate and the patterns observed with this technique.

Original languageEnglish (US)
Pages (from-to)2639-2645
Number of pages7
JournalJournal of General Microbiology
Volume138
Issue number12
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Type II Site Specific Deoxyribonucleases
DNA Restriction Enzymes
Ribosomal DNA
Polyacrylamide Gel Electrophoresis
DNA
Streptococcus suis
Swine
Digestion
Sepsis
Pneumonia
Escherichia coli

ASJC Scopus subject areas

  • Microbiology

Cite this

Molecular analysis of isolates of Streptoccocus suis capsular type 2 by restriction-endonuclease-digested DNA separated on SDS-PAGE and by hybridization with an rDNA probe. / Beaudoin, M.; Harel, J.; Higgins, Robert; Gottschalk, M.; Frenette, M.; MacInnes, J. I.

In: Journal of General Microbiology, Vol. 138, No. 12, 01.01.1992, p. 2639-2645.

Research output: Contribution to journalArticle

@article{2502a604b3af4e45bf4f7577836cd2b6,
title = "Molecular analysis of isolates of Streptoccocus suis capsular type 2 by restriction-endonuclease-digested DNA separated on SDS-PAGE and by hybridization with an rDNA probe",
abstract = "This study was undertaken to assess the discriminatory value of restriction endonuclease (RE) digestion patterns of Streptococcus suis chromosomal DNA using polyacrylamide gel electrophoresis (SDS-PAGE) and DNA-rDNA hybridization. For the RE digestion patterns, DNAs were digested separately with the enzymes BamHI and BglII and the resultant fragments were separated by SDS-PAGE. An Escherichia coli rDNA probe derived from pKK3535 was used for the hybridization. Twenty-three S. suis capsular type 2 isolates recovered from diseased and clinically healthy pigs, from a human case, and from a cow were compared in this study. The majority of isolates associated with septicaemia belonged to one restriction endonuclease analysis (REA) profile group. Isolates associated with pneumonia belonged either to the REA profile group of isolates associated with septicaemia or to a second REA profile group. The REA profiles of isolates from clinically healthy animals were more heterogeneous. The REA profile of the type 2 reference strain, S735, which was originally isolated from a pig, was very different from those of the porcine and bovine isolates but similar to the profile of the human isolate. The profiles obtained after rDNA hybridization were more homogeneous. Although different patterns were detected in the 23 isolates, there was no correlation between the source of the isolate and the patterns observed with this technique.",
author = "M. Beaudoin and J. Harel and Robert Higgins and M. Gottschalk and M. Frenette and MacInnes, {J. I.}",
year = "1992",
month = "1",
day = "1",
doi = "10.1099/00221287-138-12-2639",
language = "English (US)",
volume = "138",
pages = "2639--2645",
journal = "Microbiology (United Kingdom)",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "12",

}

TY - JOUR

T1 - Molecular analysis of isolates of Streptoccocus suis capsular type 2 by restriction-endonuclease-digested DNA separated on SDS-PAGE and by hybridization with an rDNA probe

AU - Beaudoin, M.

AU - Harel, J.

AU - Higgins, Robert

AU - Gottschalk, M.

AU - Frenette, M.

AU - MacInnes, J. I.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - This study was undertaken to assess the discriminatory value of restriction endonuclease (RE) digestion patterns of Streptococcus suis chromosomal DNA using polyacrylamide gel electrophoresis (SDS-PAGE) and DNA-rDNA hybridization. For the RE digestion patterns, DNAs were digested separately with the enzymes BamHI and BglII and the resultant fragments were separated by SDS-PAGE. An Escherichia coli rDNA probe derived from pKK3535 was used for the hybridization. Twenty-three S. suis capsular type 2 isolates recovered from diseased and clinically healthy pigs, from a human case, and from a cow were compared in this study. The majority of isolates associated with septicaemia belonged to one restriction endonuclease analysis (REA) profile group. Isolates associated with pneumonia belonged either to the REA profile group of isolates associated with septicaemia or to a second REA profile group. The REA profiles of isolates from clinically healthy animals were more heterogeneous. The REA profile of the type 2 reference strain, S735, which was originally isolated from a pig, was very different from those of the porcine and bovine isolates but similar to the profile of the human isolate. The profiles obtained after rDNA hybridization were more homogeneous. Although different patterns were detected in the 23 isolates, there was no correlation between the source of the isolate and the patterns observed with this technique.

AB - This study was undertaken to assess the discriminatory value of restriction endonuclease (RE) digestion patterns of Streptococcus suis chromosomal DNA using polyacrylamide gel electrophoresis (SDS-PAGE) and DNA-rDNA hybridization. For the RE digestion patterns, DNAs were digested separately with the enzymes BamHI and BglII and the resultant fragments were separated by SDS-PAGE. An Escherichia coli rDNA probe derived from pKK3535 was used for the hybridization. Twenty-three S. suis capsular type 2 isolates recovered from diseased and clinically healthy pigs, from a human case, and from a cow were compared in this study. The majority of isolates associated with septicaemia belonged to one restriction endonuclease analysis (REA) profile group. Isolates associated with pneumonia belonged either to the REA profile group of isolates associated with septicaemia or to a second REA profile group. The REA profiles of isolates from clinically healthy animals were more heterogeneous. The REA profile of the type 2 reference strain, S735, which was originally isolated from a pig, was very different from those of the porcine and bovine isolates but similar to the profile of the human isolate. The profiles obtained after rDNA hybridization were more homogeneous. Although different patterns were detected in the 23 isolates, there was no correlation between the source of the isolate and the patterns observed with this technique.

UR - http://www.scopus.com/inward/record.url?scp=0027074596&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027074596&partnerID=8YFLogxK

U2 - 10.1099/00221287-138-12-2639

DO - 10.1099/00221287-138-12-2639

M3 - Article

C2 - 1362584

AN - SCOPUS:0027074596

VL - 138

SP - 2639

EP - 2645

JO - Microbiology (United Kingdom)

JF - Microbiology (United Kingdom)

SN - 1350-0872

IS - 12

ER -