Molecular analysis of inorganic nitrogen assimilation in yeasts

Prabhakara V Choudary, G. Ramananda Rao

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Assimilation of nitrate and various other inorganic nitrogen compounds by different yeasts was investigated. Nitrate, nitrite, hydroxylamine, hydrazine, ammonium sulphate, urea and L-asparagine were tested as sole sources of nitrogen for the growth of Candida albicans, C. pelliculosa, Debaryomyces hansenii, Saccharomyces cerevisiae, C. tropicalis, and C. utilis. Ammonium sulphate and L-asparagine supported the growth of all the yeasts tested except D. hansenii while hydroxylamine and hydrazine failed to support the growth of any. Nitrate and nitrite were assimilated only by C. utilis. Nitrate utilization by C. utilis was also accompanied by the enzymatic activities of NAD(P)H: nitrate oxidoreductase (EC 1.6.6.2) and NAD(P)H: nitrite oxidoreductase (EC 1.6.6.4), but not reduced methyl viologen-or FAD-nitrate oxidoreductases (EC 1.7.99.4). It is demonstrated here that nitrate and nitrite reductase activities are responsible for the ability of C. utilis to assimilate primary nitrogen.

Original languageEnglish (US)
Pages (from-to)183-186
Number of pages4
JournalArchives of Microbiology
Volume138
Issue number3
DOIs
StatePublished - Jul 1984
Externally publishedYes

Keywords

  • Candida utilis
  • Food yeast
  • Nitrate reductase
  • Nitrate reduction
  • Nitrite reductase
  • Nitrogen assimilation
  • Nitrogenous oxide
  • Yeast

ASJC Scopus subject areas

  • Microbiology

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