Molecular analysis of gene expression in the developing pontocerebellar projection system

Elva D Diaz, Yongchao Ge, Yee Hwa Yang, Kenneth C. Loh, Tito A. Serafini, Yasushi Okazaki, Yoshihide Hayashizaki, Terence P. Speed, John Ngai, Peter Scheiffele

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Abstract

As an approach toward understanding the molecular mechanisms of neuronal differentiation, we utilized DNA microarrays to elucidate global patterns of gene expression during pontocerebellar development. Through this analysis, we identified groups of genes specific to neuronal precursor cells, associated with axon outgrowth, and regulated in response to contact with synaptic target cells. In the cerebellum, we identified a phase of granule cell differentiation that is independent of interactions with other cerebellar cell types. Analysis of pontine gene expression revealed that distinct programs of gene expression, correlated with axon outgrowth and synapse formation, can be decoupled and are likely influenced by different cells in the cerebellar target environment. Our approach provides insight into the genetic programs underlying the differentiation of specific cell types in the pontocerebellar projection system.

Original languageEnglish (US)
Pages (from-to)417-434
Number of pages18
JournalNeuron
Volume36
Issue number3
DOIs
StatePublished - Oct 24 2002

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Diaz, E. D., Ge, Y., Yang, Y. H., Loh, K. C., Serafini, T. A., Okazaki, Y., Hayashizaki, Y., Speed, T. P., Ngai, J., & Scheiffele, P. (2002). Molecular analysis of gene expression in the developing pontocerebellar projection system. Neuron, 36(3), 417-434. https://doi.org/10.1016/S0896-6273(02)01016-4