Purpose: To determine the ability of norepinephrine to modulate proliferation of SV-40 transformed human corneal epithelial cells. Methods: Assays were performed using SV-40 human corneal epithelial cells (Araki-Sasaki et al. 1995. IOVS 36:614-621). For proliferation assays: cells were plated in 96 well plates coated with fibronectin and collagen. A dose response curve was generated for norepinephrine in concentrations from lOpM to lOuM. Cell number in each well was evaluated using the fluorochrome Calcein AM (Molecular Probes(r)-an intracellular esterase cleavage substrate) and fluorescence determined using an automated fluorescent plate reader. Results: Norepinephrine significantly increased cell proliferation in concentrations from lOOnM to 10 uM. Conclusions: Norepinephrine is normally found in the cornea and may play significant physiologic functions as regards maintenance of a healthy anterior corneal epithelium.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - 1997|
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