Modulation of sciatic nerve expression of class 3 semaphorins by nerve injury

Jahan Ara, Peter Bannerman, Ashleigh Hahn, Sylvia Ramirez, David E Pleasure

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Beginning with the unexpected finding by cDNA array analysis that neuropilin-2 is induced in sciatic nerve distal to a transection, we document, for the first time, up-regulation in the axotomized adult peripheral nervous system of class 3 semaphorins and their receptors, which are known to play prominent roles in axonal guidance during neural development. Previously, we described the use of cDNA arrays to screen for novel peripheral nervous system axotomy-induced candidate neurotrophic proteins. A novel finding of that prior study was substantial induction of neuropilin 2 (NP2) mRNA in the axotomized nerve segments. Following up on that initial observation, we have now used real-time quantitative reverse transcription-polymerase chain reaction to demonstrate induction of genes encoding neuropilin 1 (NP1), which, like NP2, serves as a coreceptor for members of the class 3 semaphorin family of axonal guidance molecules and of five of the six known class 3 semaphorins (Sema3A, Sema3B, Sema3C, Sema3E, and Sema3F, but not Sema3D) in crushed or transected sciatic nerves.

Original languageEnglish (US)
Pages (from-to)1153-1159
Number of pages7
JournalNeurochemical Research
Volume29
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Neuropilin-2
Semaphorins
Sciatic Nerve
Peripheral Nervous System
Modulation
Neurology
Oligonucleotide Array Sequence Analysis
Wounds and Injuries
Complementary DNA
Neuropilin-1
Axotomy
Gene encoding
Polymerase chain reaction
Nerve Growth Factors
Transcription
Reverse Transcription
Up-Regulation
Observation
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Neuropilin
  • Real-time reverse transcripiton-polymerase chain reaction
  • Sciatic nerve
  • Semaphorin
  • Wallerian degeneration

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Modulation of sciatic nerve expression of class 3 semaphorins by nerve injury. / Ara, Jahan; Bannerman, Peter; Hahn, Ashleigh; Ramirez, Sylvia; Pleasure, David E.

In: Neurochemical Research, Vol. 29, No. 6, 06.2004, p. 1153-1159.

Research output: Contribution to journalArticle

Ara, Jahan ; Bannerman, Peter ; Hahn, Ashleigh ; Ramirez, Sylvia ; Pleasure, David E. / Modulation of sciatic nerve expression of class 3 semaphorins by nerve injury. In: Neurochemical Research. 2004 ; Vol. 29, No. 6. pp. 1153-1159.
@article{fee84c9c43a9408191d99ee55b1afb66,
title = "Modulation of sciatic nerve expression of class 3 semaphorins by nerve injury",
abstract = "Beginning with the unexpected finding by cDNA array analysis that neuropilin-2 is induced in sciatic nerve distal to a transection, we document, for the first time, up-regulation in the axotomized adult peripheral nervous system of class 3 semaphorins and their receptors, which are known to play prominent roles in axonal guidance during neural development. Previously, we described the use of cDNA arrays to screen for novel peripheral nervous system axotomy-induced candidate neurotrophic proteins. A novel finding of that prior study was substantial induction of neuropilin 2 (NP2) mRNA in the axotomized nerve segments. Following up on that initial observation, we have now used real-time quantitative reverse transcription-polymerase chain reaction to demonstrate induction of genes encoding neuropilin 1 (NP1), which, like NP2, serves as a coreceptor for members of the class 3 semaphorin family of axonal guidance molecules and of five of the six known class 3 semaphorins (Sema3A, Sema3B, Sema3C, Sema3E, and Sema3F, but not Sema3D) in crushed or transected sciatic nerves.",
keywords = "Neuropilin, Real-time reverse transcripiton-polymerase chain reaction, Sciatic nerve, Semaphorin, Wallerian degeneration",
author = "Jahan Ara and Peter Bannerman and Ashleigh Hahn and Sylvia Ramirez and Pleasure, {David E}",
year = "2004",
month = "6",
doi = "10.1023/B:NERE.0000023602.72354.82",
language = "English (US)",
volume = "29",
pages = "1153--1159",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Modulation of sciatic nerve expression of class 3 semaphorins by nerve injury

AU - Ara, Jahan

AU - Bannerman, Peter

AU - Hahn, Ashleigh

AU - Ramirez, Sylvia

AU - Pleasure, David E

PY - 2004/6

Y1 - 2004/6

N2 - Beginning with the unexpected finding by cDNA array analysis that neuropilin-2 is induced in sciatic nerve distal to a transection, we document, for the first time, up-regulation in the axotomized adult peripheral nervous system of class 3 semaphorins and their receptors, which are known to play prominent roles in axonal guidance during neural development. Previously, we described the use of cDNA arrays to screen for novel peripheral nervous system axotomy-induced candidate neurotrophic proteins. A novel finding of that prior study was substantial induction of neuropilin 2 (NP2) mRNA in the axotomized nerve segments. Following up on that initial observation, we have now used real-time quantitative reverse transcription-polymerase chain reaction to demonstrate induction of genes encoding neuropilin 1 (NP1), which, like NP2, serves as a coreceptor for members of the class 3 semaphorin family of axonal guidance molecules and of five of the six known class 3 semaphorins (Sema3A, Sema3B, Sema3C, Sema3E, and Sema3F, but not Sema3D) in crushed or transected sciatic nerves.

AB - Beginning with the unexpected finding by cDNA array analysis that neuropilin-2 is induced in sciatic nerve distal to a transection, we document, for the first time, up-regulation in the axotomized adult peripheral nervous system of class 3 semaphorins and their receptors, which are known to play prominent roles in axonal guidance during neural development. Previously, we described the use of cDNA arrays to screen for novel peripheral nervous system axotomy-induced candidate neurotrophic proteins. A novel finding of that prior study was substantial induction of neuropilin 2 (NP2) mRNA in the axotomized nerve segments. Following up on that initial observation, we have now used real-time quantitative reverse transcription-polymerase chain reaction to demonstrate induction of genes encoding neuropilin 1 (NP1), which, like NP2, serves as a coreceptor for members of the class 3 semaphorin family of axonal guidance molecules and of five of the six known class 3 semaphorins (Sema3A, Sema3B, Sema3C, Sema3E, and Sema3F, but not Sema3D) in crushed or transected sciatic nerves.

KW - Neuropilin

KW - Real-time reverse transcripiton-polymerase chain reaction

KW - Sciatic nerve

KW - Semaphorin

KW - Wallerian degeneration

UR - http://www.scopus.com/inward/record.url?scp=3142658610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142658610&partnerID=8YFLogxK

U2 - 10.1023/B:NERE.0000023602.72354.82

DO - 10.1023/B:NERE.0000023602.72354.82

M3 - Article

VL - 29

SP - 1153

EP - 1159

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 6

ER -