Modulation of murine mammary tumor vasculature by dietary n-3 fatty acids in fish oil

Mithia Mukutmoni-Norris, Neil Hubbard, Kent L Erickson

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have previously shown that mice fed a high (n-3) fatty acid- containing diet with 20% (w/w) total fat had significantly slower mammary tumor growth, decreased numbers of metastatic pulmonary nodules, and decreased total metastatic load. In this study we sought to determine whether tumor vascularization was altered in mice fed diets varying in concentrations of (n-3) and (n-6) fatty acids. Several direct or indirect parameters of vascularization were tested. With 20% dietary fat, fish oil (FO) or a mixture of FO and safflower oil (FS) significantly reduced blood vascular area, mast cell number and macrophage infiltration in solid mammary tumors compared to tumors grown in mice fed safflower oil (SO). A decreasing trend was seen in the percent area of vessels positive for CD31 and vascular endothelial growth factor (VEGF) in the 20% FO and 20% FS compared to the 20% SO dietary groups. VEGF concentrations were twice as high in smaller tumors (100 mm3) from all dietary groups as compared to larger tumors (500 mm3). A two-fold increase in VEGF levels was found in the 20% SO dietary group compared to the 20% FO group in 100-mm3 but not larger tumors. We conclude that at 20% total fat, the n-3 fatty acids found in fish oil may inhibit primary mammary tumor growth through modulation of select determinants of vascularization. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)101-109
Number of pages9
JournalCancer Letters
Volume150
Issue number1
DOIs
StatePublished - Mar 13 2000

Fingerprint

Fish Oils
Safflower Oil
Omega-3 Fatty Acids
Breast Neoplasms
Vascular Endothelial Growth Factor A
Neoplasms
Fats
Diet
Dietary Fats
Growth
Mast Cells
Blood Vessels
Fatty Acids
Cell Count
Macrophages
Lung

Keywords

  • Blood vasculature
  • Mammary tumor
  • n-3/n-6 Fatty acid
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Modulation of murine mammary tumor vasculature by dietary n-3 fatty acids in fish oil. / Mukutmoni-Norris, Mithia; Hubbard, Neil; Erickson, Kent L.

In: Cancer Letters, Vol. 150, No. 1, 13.03.2000, p. 101-109.

Research output: Contribution to journalArticle

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