Modulation of blood cell gene expression by DHA supplementation in hypertriglyceridemic men

Kevin Dawson, Ling Zhao, Yuriko Adkins, Madhuri Vemuri, Raymond L. Rodriguez, Jeffrey Gregg, Darshan S. Kelley, Daniel H. Hwang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Our previous study with docosahexaenoic acid (DHA) supplementation to hypertriglyceridemic men showed that DHA reduced several risk factors for cardiovascular disease, including the plasma concentration of inflammatory markers. To determine the effect of DHA supplementation on the global gene expression pattern, we performed Affymetrix GeneChip microarray analysis of blood cells [treated with lipopolysaccharide (LPS) or vehicle] drawn before and after the supplementation of DHA from the hypertriglyceridemic men who participated in that study. Genes that were significantly differentially regulated by the LPS treatment and DHA supplementation were identified. Differential regulation of 18 genes was then verified by quantitative real-time polymerase chain reaction (qRT-PCR). Both microarray and qRT-PCR data showed that DHA supplementation significantly suppressed the expression of low-density lipoprotein (LDL) receptor and cathepsin L1, both of which were also up-regulated by LPS. DHA supplementation also suppressed oxidized LDL (lectin-like) receptor 1 (OLR1). However, LPS did not induce OLR1 mRNA expression. Enrichment with Gene Ontology categories demonstrated that the genes related to transcription factor activity, immunity, host defense and inflammatory responses were inversely regulated by LPS and DHA. These results provide supporting evidence for the anti-inflammatory effects of DHA supplementation, and reveal previously unrecognized genes that are regulated by DHA and are associated with risk factors of cardiovascular diseases.

Original languageEnglish (US)
Pages (from-to)616-621
Number of pages6
JournalJournal of Nutritional Biochemistry
Volume23
Issue number6
DOIs
StatePublished - Jun 2012

Fingerprint

Docosahexaenoic Acids
Gene expression
Blood Cells
Blood
Cells
Modulation
Gene Expression
Lipopolysaccharides
Genes
Polymerase chain reaction
Microarrays
Real-Time Polymerase Chain Reaction
Class E Scavenger Receptors
Cardiovascular Diseases
Cathepsins
Gene Ontology
LDL Receptors
Microarray Analysis
Ontology
Immunity

Keywords

  • Cardiovascular disease
  • Cathepsin L1
  • Docosahexaenoic acid
  • Gene expression
  • Inflammation
  • LDL receptor
  • Oxidized LDL receptor

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Modulation of blood cell gene expression by DHA supplementation in hypertriglyceridemic men. / Dawson, Kevin; Zhao, Ling; Adkins, Yuriko; Vemuri, Madhuri; Rodriguez, Raymond L.; Gregg, Jeffrey; Kelley, Darshan S.; Hwang, Daniel H.

In: Journal of Nutritional Biochemistry, Vol. 23, No. 6, 06.2012, p. 616-621.

Research output: Contribution to journalArticle

Dawson, K, Zhao, L, Adkins, Y, Vemuri, M, Rodriguez, RL, Gregg, J, Kelley, DS & Hwang, DH 2012, 'Modulation of blood cell gene expression by DHA supplementation in hypertriglyceridemic men', Journal of Nutritional Biochemistry, vol. 23, no. 6, pp. 616-621. https://doi.org/10.1016/j.jnutbio.2011.03.004
Dawson, Kevin ; Zhao, Ling ; Adkins, Yuriko ; Vemuri, Madhuri ; Rodriguez, Raymond L. ; Gregg, Jeffrey ; Kelley, Darshan S. ; Hwang, Daniel H. / Modulation of blood cell gene expression by DHA supplementation in hypertriglyceridemic men. In: Journal of Nutritional Biochemistry. 2012 ; Vol. 23, No. 6. pp. 616-621.
@article{001a5c51bb1a4e9399ce2a3e4bdf418b,
title = "Modulation of blood cell gene expression by DHA supplementation in hypertriglyceridemic men",
abstract = "Our previous study with docosahexaenoic acid (DHA) supplementation to hypertriglyceridemic men showed that DHA reduced several risk factors for cardiovascular disease, including the plasma concentration of inflammatory markers. To determine the effect of DHA supplementation on the global gene expression pattern, we performed Affymetrix GeneChip microarray analysis of blood cells [treated with lipopolysaccharide (LPS) or vehicle] drawn before and after the supplementation of DHA from the hypertriglyceridemic men who participated in that study. Genes that were significantly differentially regulated by the LPS treatment and DHA supplementation were identified. Differential regulation of 18 genes was then verified by quantitative real-time polymerase chain reaction (qRT-PCR). Both microarray and qRT-PCR data showed that DHA supplementation significantly suppressed the expression of low-density lipoprotein (LDL) receptor and cathepsin L1, both of which were also up-regulated by LPS. DHA supplementation also suppressed oxidized LDL (lectin-like) receptor 1 (OLR1). However, LPS did not induce OLR1 mRNA expression. Enrichment with Gene Ontology categories demonstrated that the genes related to transcription factor activity, immunity, host defense and inflammatory responses were inversely regulated by LPS and DHA. These results provide supporting evidence for the anti-inflammatory effects of DHA supplementation, and reveal previously unrecognized genes that are regulated by DHA and are associated with risk factors of cardiovascular diseases.",
keywords = "Cardiovascular disease, Cathepsin L1, Docosahexaenoic acid, Gene expression, Inflammation, LDL receptor, Oxidized LDL receptor",
author = "Kevin Dawson and Ling Zhao and Yuriko Adkins and Madhuri Vemuri and Rodriguez, {Raymond L.} and Jeffrey Gregg and Kelley, {Darshan S.} and Hwang, {Daniel H.}",
year = "2012",
month = "6",
doi = "10.1016/j.jnutbio.2011.03.004",
language = "English (US)",
volume = "23",
pages = "616--621",
journal = "Journal of Nutritional Biochemistry",
issn = "0955-2863",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Modulation of blood cell gene expression by DHA supplementation in hypertriglyceridemic men

AU - Dawson, Kevin

AU - Zhao, Ling

AU - Adkins, Yuriko

AU - Vemuri, Madhuri

AU - Rodriguez, Raymond L.

AU - Gregg, Jeffrey

AU - Kelley, Darshan S.

AU - Hwang, Daniel H.

PY - 2012/6

Y1 - 2012/6

N2 - Our previous study with docosahexaenoic acid (DHA) supplementation to hypertriglyceridemic men showed that DHA reduced several risk factors for cardiovascular disease, including the plasma concentration of inflammatory markers. To determine the effect of DHA supplementation on the global gene expression pattern, we performed Affymetrix GeneChip microarray analysis of blood cells [treated with lipopolysaccharide (LPS) or vehicle] drawn before and after the supplementation of DHA from the hypertriglyceridemic men who participated in that study. Genes that were significantly differentially regulated by the LPS treatment and DHA supplementation were identified. Differential regulation of 18 genes was then verified by quantitative real-time polymerase chain reaction (qRT-PCR). Both microarray and qRT-PCR data showed that DHA supplementation significantly suppressed the expression of low-density lipoprotein (LDL) receptor and cathepsin L1, both of which were also up-regulated by LPS. DHA supplementation also suppressed oxidized LDL (lectin-like) receptor 1 (OLR1). However, LPS did not induce OLR1 mRNA expression. Enrichment with Gene Ontology categories demonstrated that the genes related to transcription factor activity, immunity, host defense and inflammatory responses were inversely regulated by LPS and DHA. These results provide supporting evidence for the anti-inflammatory effects of DHA supplementation, and reveal previously unrecognized genes that are regulated by DHA and are associated with risk factors of cardiovascular diseases.

AB - Our previous study with docosahexaenoic acid (DHA) supplementation to hypertriglyceridemic men showed that DHA reduced several risk factors for cardiovascular disease, including the plasma concentration of inflammatory markers. To determine the effect of DHA supplementation on the global gene expression pattern, we performed Affymetrix GeneChip microarray analysis of blood cells [treated with lipopolysaccharide (LPS) or vehicle] drawn before and after the supplementation of DHA from the hypertriglyceridemic men who participated in that study. Genes that were significantly differentially regulated by the LPS treatment and DHA supplementation were identified. Differential regulation of 18 genes was then verified by quantitative real-time polymerase chain reaction (qRT-PCR). Both microarray and qRT-PCR data showed that DHA supplementation significantly suppressed the expression of low-density lipoprotein (LDL) receptor and cathepsin L1, both of which were also up-regulated by LPS. DHA supplementation also suppressed oxidized LDL (lectin-like) receptor 1 (OLR1). However, LPS did not induce OLR1 mRNA expression. Enrichment with Gene Ontology categories demonstrated that the genes related to transcription factor activity, immunity, host defense and inflammatory responses were inversely regulated by LPS and DHA. These results provide supporting evidence for the anti-inflammatory effects of DHA supplementation, and reveal previously unrecognized genes that are regulated by DHA and are associated with risk factors of cardiovascular diseases.

KW - Cardiovascular disease

KW - Cathepsin L1

KW - Docosahexaenoic acid

KW - Gene expression

KW - Inflammation

KW - LDL receptor

KW - Oxidized LDL receptor

UR - http://www.scopus.com/inward/record.url?scp=84860889282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860889282&partnerID=8YFLogxK

U2 - 10.1016/j.jnutbio.2011.03.004

DO - 10.1016/j.jnutbio.2011.03.004

M3 - Article

C2 - 21775114

AN - SCOPUS:84860889282

VL - 23

SP - 616

EP - 621

JO - Journal of Nutritional Biochemistry

JF - Journal of Nutritional Biochemistry

SN - 0955-2863

IS - 6

ER -