Modular microinstrumentation for endothelial cell research

Bonnie L. Gray; Abdul I. Barakat; Deborah Lieu; Scott D. Collins; Rosemary L. Smith

Modular microinstrumentation for endothelial cell research

Bonnie L. Gray, Abdul I. Barakat, Deborah Lieu, Scott D. Collins, Rosemary L. Smith

Cardiovascular Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

Microfabrication technology is implemented to realize a fluidic microinstrument for the study of endothelial cell elongation and cell responsiveness to fluid flow. The microinstrument contains arrays of microchannels, 30-300 μm wide, that are fabricated by deep reactive ion etching (DRIE) of silicon and anodic bonding to glass. Silicon fluidic input/output modules, also micromachined in silicon, provide modular connections between the microchannels and off-chip devices for flow monitoring and control. Image analysis of cells cultured in microchannels shows that the cells become progressively more elongated as channel width decreases. When subjected to a fluid shear stress of 2 N/m ², cuboidal cells grown in 200 μm wide microchannels progressively align and elongate in the direction of flow.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Publisher	Society of Photo-Optical Instrumentation Engineers
Pages	88-94
Number of pages	7
Volume	3912
State	Published - 2000
Event	Micro- and Nanotechnology for Biomedical and Environmental Applications - San Jose, CA, USA Duration: Jan 26 2000 → Jan 27 2000

Other

Other	Micro- and Nanotechnology for Biomedical and Environmental Applications
City	San Jose, CA, USA
Period	1/26/00 → 1/27/00

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

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Cite this

Gray, B. L., Barakat, A. I., Lieu, D., Collins, S. D., & Smith, R. L. (2000). Modular microinstrumentation for endothelial cell research. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3912, pp. 88-94). Society of Photo-Optical Instrumentation Engineers.

Modular microinstrumentation for endothelial cell research. / Gray, Bonnie L.; Barakat, Abdul I.; Lieu, Deborah; Collins, Scott D.; Smith, Rosemary L.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3912 Society of Photo-Optical Instrumentation Engineers, 2000. p. 88-94.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Gray, BL, Barakat, AI, Lieu, D, Collins, SD & Smith, RL 2000, Modular microinstrumentation for endothelial cell research. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3912, Society of Photo-Optical Instrumentation Engineers, pp. 88-94, Micro- and Nanotechnology for Biomedical and Environmental Applications, San Jose, CA, USA, 1/26/00.

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