Modular microinstrumentation for endothelial cell research

Bonnie L. Gray, Abdul I. Barakat, Deborah Lieu, Scott D. Collins, Rosemary L. Smith

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Microfabrication technology is implemented to realize a fluidic microinstrument for the study of endothelial cell elongation and cell responsiveness to fluid flow. The microinstrument contains arrays of microchannels, 30-300 μm wide, that are fabricated by deep reactive ion etching (DRIE) of silicon and anodic bonding to glass. Silicon fluidic input/output modules, also micromachined in silicon, provide modular connections between the microchannels and off-chip devices for flow monitoring and control. Image analysis of cells cultured in microchannels shows that the cells become progressively more elongated as channel width decreases. When subjected to a fluid shear stress of 2 N/m 2, cuboidal cells grown in 200 μm wide microchannels progressively align and elongate in the direction of flow.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages88-94
Number of pages7
Volume3912
StatePublished - 2000
EventMicro- and Nanotechnology for Biomedical and Environmental Applications - San Jose, CA, USA
Duration: Jan 26 2000Jan 27 2000

Other

OtherMicro- and Nanotechnology for Biomedical and Environmental Applications
CitySan Jose, CA, USA
Period1/26/001/27/00

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Fingerprint Dive into the research topics of 'Modular microinstrumentation for endothelial cell research'. Together they form a unique fingerprint.

  • Cite this

    Gray, B. L., Barakat, A. I., Lieu, D., Collins, S. D., & Smith, R. L. (2000). Modular microinstrumentation for endothelial cell research. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3912, pp. 88-94). Society of Photo-Optical Instrumentation Engineers.