Modification of the pig CFTR gene mediated by small fragment homologous replacement

Rosalie Maurisse, Judy Cheung, Jonathan Widdicombe, Dieter C. Gruenert

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Scopus citations

Abstract

The generation of a pig model of cystic fibrosis (CF) is a multistep process. Initial steps in this process involved the design and cloning of a small DNA fragment (SDF) or large oligodeoxynucleotide (LODN) that contains the F508del mutation and a silent restriction fragment length polymorphism causing mutation. This SDF/LODN was transfected into wild-type (WT) pig fetal fibroblast with the intention of modifying the pig genomic DNA by small fragment homologous replacement (SFHR). The targeted deletion (F508del) was detected in a subpopulation of transfected cells by allele-specific polymerase chain reaction (AS-PCR).

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages120-123
Number of pages4
Volume1082
DOIs
StatePublished - Oct 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1082
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • CFTR
  • Gene modification
  • SFHR
  • Site-directed mutagenesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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