Modification of the pig CFTR gene mediated by small fragment homologous replacement

Rosalie Maurisse, Judy Cheung, Jonathan Widdicombe, Dieter C. Gruenert

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

The generation of a pig model of cystic fibrosis (CF) is a multistep process. Initial steps in this process involved the design and cloning of a small DNA fragment (SDF) or large oligodeoxynucleotide (LODN) that contains the F508del mutation and a silent restriction fragment length polymorphism causing mutation. This SDF/LODN was transfected into wild-type (WT) pig fetal fibroblast with the intention of modifying the pig genomic DNA by small fragment homologous replacement (SFHR). The targeted deletion (F508del) was detected in a subpopulation of transfected cells by allele-specific polymerase chain reaction (AS-PCR).

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages120-123
Number of pages4
Volume1082
DOIs
StatePublished - Oct 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1082
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Swine
Genes
Oligodeoxyribonucleotides
DNA
Cloning
Polymerase chain reaction
Fibroblasts
Polymorphism
Cystic Fibrosis
Restriction Fragment Length Polymorphisms
Organism Cloning
Alleles
Cells
Polymerase Chain Reaction
Mutation
Silent Mutation

Keywords

  • CFTR
  • Gene modification
  • SFHR
  • Site-directed mutagenesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Maurisse, R., Cheung, J., Widdicombe, J., & Gruenert, D. C. (2006). Modification of the pig CFTR gene mediated by small fragment homologous replacement. In Annals of the New York Academy of Sciences (Vol. 1082, pp. 120-123). (Annals of the New York Academy of Sciences; Vol. 1082). https://doi.org/10.1196/annals.1348.063

Modification of the pig CFTR gene mediated by small fragment homologous replacement. / Maurisse, Rosalie; Cheung, Judy; Widdicombe, Jonathan; Gruenert, Dieter C.

Annals of the New York Academy of Sciences. Vol. 1082 2006. p. 120-123 (Annals of the New York Academy of Sciences; Vol. 1082).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Maurisse, R, Cheung, J, Widdicombe, J & Gruenert, DC 2006, Modification of the pig CFTR gene mediated by small fragment homologous replacement. in Annals of the New York Academy of Sciences. vol. 1082, Annals of the New York Academy of Sciences, vol. 1082, pp. 120-123. https://doi.org/10.1196/annals.1348.063
Maurisse R, Cheung J, Widdicombe J, Gruenert DC. Modification of the pig CFTR gene mediated by small fragment homologous replacement. In Annals of the New York Academy of Sciences. Vol. 1082. 2006. p. 120-123. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1348.063
Maurisse, Rosalie ; Cheung, Judy ; Widdicombe, Jonathan ; Gruenert, Dieter C. / Modification of the pig CFTR gene mediated by small fragment homologous replacement. Annals of the New York Academy of Sciences. Vol. 1082 2006. pp. 120-123 (Annals of the New York Academy of Sciences).
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