Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation

A. Z. Reznick, Carroll E Cross, M. L. Hu, Y. J. Suzuki, S. Khwaja, A. Safadi, P. A. Motchnik, L. Packer, B. Halliwell

Research output: Contribution to journalArticle

240 Scopus citations

Abstract

Exposure of human plasma to gas-phase (but not to whole) cigarette smoke (CS) produces oxidative damage to lipids [Frei, Forte, Ames and Cross (1991) Biochem. J. 277, 133-138], which is prevented by ascorbic acid. The ability of CS to induce protein damage was measured by the carbonyl assay and by loss of enzyme activity and protein -SH groups. Both whole and gas-phase CS caused formation of carbonyls in human plasma, which was partially inhibited by GSH but not by ascorbic acid or metal-ion-chelating agents. Isolated albumin exposed to CS showed much faster carbonyl formation (per unit protein) than did whole plasma; damage to isolated albumin was partially prevented by chelating agents. Isolated creatine kinase (CK) lost activity upon exposure to CS much faster than did CK in plasma. Direct addition to plasma of mixtures of some or all of the aldehydes reported to be present in CS caused protein carbonyl formation and inactivation of CK, but neither occurred to the extent produced by CS exposure.

Original languageEnglish (US)
Pages (from-to)607-611
Number of pages5
JournalBiochemical Journal
Volume286
Issue number2
StatePublished - 1992

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation'. Together they form a unique fingerprint.

  • Cite this

    Reznick, A. Z., Cross, C. E., Hu, M. L., Suzuki, Y. J., Khwaja, S., Safadi, A., Motchnik, P. A., Packer, L., & Halliwell, B. (1992). Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation. Biochemical Journal, 286(2), 607-611.