Moderate hyperoxia (40%) increases antioxidant levels in mouse tissue

Eugene S Lee, W. Ed Smith, Hung T. Quach, Bryan D. Jones, Steven M. Santilli, Govind T. Vatassery

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Background. Oxygen is routinely administered to patients to improve clinical outcome. Since studies have shown that administering 100% oxygen can cause unwanted side effects, intermediate concentrations of 40% oxygen are used in clinical practice. In this study, we examined whether the breathing of 40% oxygen causes beneficial effects upon tissue levels of antioxidants such as vitamin E, vitamin C, and glutathione. Methods. Four-month-old mice were separated into two groups: control (n = 11) and experimental (n = 11). The treatment group was administered 40% oxygen for 10 days. Brain, heart, lung, liver, testes, and skeletal muscle were harvested and tissue antioxidant levels were determined by HPLC. Results. Vitamin E concentrations were higher in brain, heart, lung, liver, and testes of the treatment group (P < 0.05). Glutathione concentrations were higher in the lung tissue only (P < 0.05). No differences were found in vitamin C levels. Conclusions. The data suggest that mice respond to oxidative stress by increasing tissue vitamin E incorporation and cellular synthesis of glutathione in the lung when exposed to moderate levels (40%) of hyperoxia.

Original languageEnglish (US)
Pages (from-to)80-84
Number of pages5
JournalJournal of Surgical Research
Issue number2
StatePublished - Aug 2005


  • Antioxidants
  • Glutathione
  • Hyperoxia
  • Oxidative stress
  • Vitamin E

ASJC Scopus subject areas

  • Surgery


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