Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes

Robert A. Jacob, Giovanna M. Aiello, Charles B. Stephensen, Jeffrey B. Blumberg, Paul E. Milbury, Lynn M. Wallock, Bruce N. Ames

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The link between high fruit/vegetable intake and reduced chronic disease may be partly explained by antioxidant protection. To determine the effect of moderate antioxidant intake on biomarkers of oxidant damage, we assessed in vivo lipid and protein oxidation in 77 healthy men whose typical diet contained few fruits and vegetables (mean of 2.6 servings/d). The 39 nonsmokers and 38 smokers, age 20- 51 y, were given a daily supplement (272 mg vitamin C, 31 mg all-rac-α-tocopherol, and 400 μg folic acid), or placebo, for 90 d with their usual diet. Blood and urine were taken at baseline and the end of the study for determination of lipid peroxidation products, including F2-total and 8-isoprostanes, and protein carbonyls. Urine thiobarbituric acid reactive substances (TBARS) was the only oxidant damage marker that was significantly higher in smokers compared to nonsmokers (P < 0.05). Supplementation increased plasma ascorbate and tocopherol, but had no effect on the oxidant biomarkers. In healthy young men, the endogenous antioxidant defense system and a modest intake of dietary antioxidants are adequate to minimize levels of in vivo oxidant damage such that they cannot be differentiated by current methods.

Original languageEnglish (US)
Pages (from-to)740-743
Number of pages4
JournalJournal of Nutrition
Volume133
Issue number3
StatePublished - Mar 1 2003

Fingerprint

vegetable consumption
fruit consumption
Oxidants
oxidants
Vegetables
Fruit
biomarkers
Antioxidants
Biomarkers
antioxidants
8-epi-prostaglandin F2alpha
Tocopherols
tocopherols
urine
Urine
Diet
fruits
Thiobarbituric Acid Reactive Substances
chronic diseases
Folic Acid

Keywords

  • Antioxidant
  • Biomarkers
  • Fruits and vegetables
  • Human oxidant damage

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Jacob, R. A., Aiello, G. M., Stephensen, C. B., Blumberg, J. B., Milbury, P. E., Wallock, L. M., & Ames, B. N. (2003). Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes. Journal of Nutrition, 133(3), 740-743.

Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes. / Jacob, Robert A.; Aiello, Giovanna M.; Stephensen, Charles B.; Blumberg, Jeffrey B.; Milbury, Paul E.; Wallock, Lynn M.; Ames, Bruce N.

In: Journal of Nutrition, Vol. 133, No. 3, 01.03.2003, p. 740-743.

Research output: Contribution to journalArticle

Jacob, RA, Aiello, GM, Stephensen, CB, Blumberg, JB, Milbury, PE, Wallock, LM & Ames, BN 2003, 'Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes', Journal of Nutrition, vol. 133, no. 3, pp. 740-743.
Jacob RA, Aiello GM, Stephensen CB, Blumberg JB, Milbury PE, Wallock LM et al. Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes. Journal of Nutrition. 2003 Mar 1;133(3):740-743.
Jacob, Robert A. ; Aiello, Giovanna M. ; Stephensen, Charles B. ; Blumberg, Jeffrey B. ; Milbury, Paul E. ; Wallock, Lynn M. ; Ames, Bruce N. / Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes. In: Journal of Nutrition. 2003 ; Vol. 133, No. 3. pp. 740-743.
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