Model of polarization and bistability of cell fragments

Michael M. Kozlov, Alex Mogilner

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Directed cell motility is preceded by cell polarization - development of a front-rear asymmetry of the cytoskeleton and the cell shape. Extensive studies implicated complex spatial-temporal feedbacks between multiple signaling pathways in establishing cell polarity, yet physical mechanisms of this phenomenon remain elusive. Based on observations of lamellipodial fragments of fish keratocyte cells, we suggest a purely thermodynamic (not involving signaling) quantitative model of the cell polarization and bistability. The model is based on the interplay between pushing force exerted by F-actin polymerization on the cell edges, contractile force powered by myosin II across the cell, and elastic tension in the cell membrane. We calculate the thermodynamic work produced by these intracellular forces, and show that on the short timescale, the cell mechanics can be characterized by an effective energy profile with two minima that describe two stable states separated by an energy barrier and corresponding to the nonpolarized and polarized cells. Cell dynamics implied by this energy profile is bistable - the cell is either disk-shaped and stationary, or crescent-shaped and motile - with a possible transition between them upon a finite external stimulus able to drive the system over the macroscopic energy barrier. The model accounts for the observations of the keratocyte fragments' behavior and generates quantitative predictions about relations between the intracellular forces' magnitudes and the cell geometry and motility.

Original languageEnglish (US)
Pages (from-to)3811-3819
Number of pages9
JournalBiophysical Journal
Volume93
Issue number11
DOIs
StatePublished - Dec 1 2007

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Thermodynamics
Cell Movement
Physical Phenomena
Myosin Type II
Cell Polarity
Cell Shape
Mechanics
Cytoskeleton
Polymerization
Actins
Fishes
Cell Membrane
Drive

ASJC Scopus subject areas

  • Biophysics

Cite this

Model of polarization and bistability of cell fragments. / Kozlov, Michael M.; Mogilner, Alex.

In: Biophysical Journal, Vol. 93, No. 11, 01.12.2007, p. 3811-3819.

Research output: Contribution to journalArticle

Kozlov, Michael M. ; Mogilner, Alex. / Model of polarization and bistability of cell fragments. In: Biophysical Journal. 2007 ; Vol. 93, No. 11. pp. 3811-3819.
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