Model of chromosome motility in Drosophila embryos

Adaptation of a general mechanism for rapid mitosis

G. Civelekoglu-Scholey, D. J. Sharp, A. Mogilner, J. M. Scholey

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

During mitosis, ensembles of dynamic MTs and motors exert forces that coordinate chromosome segregation. Typically, chromosomes align at the metaphase spindle equator where they oscillate along the pole-pole axis before disjoining and moving poleward during anaphase A, but spindles in different cell types display differences in MT dynamicity, in the amplitude of chromosome oscillations and in rates of chromatid-to-pole motion. Drosophila embryonic mitotic spindles, for example, display remarkably dynamic MTs, barely detectable metaphase chromosome oscillations, and a rapid rate of "flux-pacman- dependent" anaphase chromatid-to-pole motility. Here we develop a force-balance model that describes Drosophila embryo chromosome motility in terms of a balance of forces acting on kinetochores and kMTs that is generated by multiple polymer ratchets and mitotic motors coupled to tension-dependent kMT dynamics. The model shows that i), multiple MTs displaying high dynamic instability can drive steady and rapid chromosome motion; ii), chromosome motility during metaphase and anaphase A can be described by a single mechanism; iii), high kinetochore dynein activity is deployed to dampen metaphase oscillations, to augment the basic flux-pacman mechanism, and to drive rapid anaphase A; iv), modulation of the MT rescue frequency by the kinetochore-associated kinesin-13 depolymerase promotes metaphase chromosome oscillations; and v), this basic mechanism can be adapted to a broad range of spindles.

Original languageEnglish (US)
Pages (from-to)3966-3982
Number of pages17
JournalBiophysical Journal
Volume90
Issue number11
DOIs
StatePublished - Jun 2006

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Mitosis
Drosophila
Embryonic Structures
Chromosomes
Metaphase
Anaphase
Kinetochores
Chromatids
Dyneins
Kinesin
Chromosome Segregation
Spindle Apparatus
Polymers

ASJC Scopus subject areas

  • Biophysics

Cite this

Model of chromosome motility in Drosophila embryos : Adaptation of a general mechanism for rapid mitosis. / Civelekoglu-Scholey, G.; Sharp, D. J.; Mogilner, A.; Scholey, J. M.

In: Biophysical Journal, Vol. 90, No. 11, 06.2006, p. 3966-3982.

Research output: Contribution to journalArticle

Civelekoglu-Scholey, G. ; Sharp, D. J. ; Mogilner, A. ; Scholey, J. M. / Model of chromosome motility in Drosophila embryos : Adaptation of a general mechanism for rapid mitosis. In: Biophysical Journal. 2006 ; Vol. 90, No. 11. pp. 3966-3982.
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