Mob1, an essential yeast gene required for completion of mitosis and maintenance of ploidy

Francis C. Luca, Mark Winey

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Mob1p is an essential Saccharomyces cerevisiae protein, identified from a two-hybrid screen, that binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob1p contains no known structural motifs; however MOB1 is a member of a conserved gene family and shares sequence similarity with a nonessential yeast gene, MOB2. Mob1p is a phosphoprotein in vivo and a substrate for the Mps1p kinase in vitro. Conditional alleles of MOB1 cause a late nuclear division arrest at restrictive temperature. MOB1 exhibits genetic interaction with three other yeast genes required for the completion of mitosis, LTE1, CDC5, and CDC15 (the latter two encode essential protein kinases). Most haploid mutant mob1 strains also display a complete increase in ploidy at permissive temperature. The mechanism for the increase in ploidy may occur through MPS1 function. One mob1 strain, which maintains stable haploidy at both permissive and restrictive temperature, diploidizes at permissive temperature when combined with the mps1-1 mutation. Strains containing mob2Δ also display a complete increase in ploidy when combined with the mps1-1 mutation. Perhaps in addition to, or as part of, its essential function in late mitosis, MOB1 is required for a cell cycle reset function necessary for the initiation of the spindle pole body duplication.

Original languageEnglish (US)
Pages (from-to)29-46
Number of pages18
JournalMolecular Biology of the Cell
Volume9
Issue number1
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Ploidies
Essential Genes
Mitosis
Yeasts
Spindle Pole Bodies
Maintenance
Temperature
Haploidy
Protein Kinases
M Phase Cell Cycle Checkpoints
Genes
Cell Nucleus Division
Saccharomyces cerevisiae Proteins
Mutation
Phosphoproteins
Cell Cycle
Phosphotransferases
Alleles

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Mob1, an essential yeast gene required for completion of mitosis and maintenance of ploidy. / Luca, Francis C.; Winey, Mark.

In: Molecular Biology of the Cell, Vol. 9, No. 1, 01.01.1998, p. 29-46.

Research output: Contribution to journalArticle

@article{e6aa8bbb958a4b728806ad912a4fc80e,
title = "Mob1, an essential yeast gene required for completion of mitosis and maintenance of ploidy",
abstract = "Mob1p is an essential Saccharomyces cerevisiae protein, identified from a two-hybrid screen, that binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob1p contains no known structural motifs; however MOB1 is a member of a conserved gene family and shares sequence similarity with a nonessential yeast gene, MOB2. Mob1p is a phosphoprotein in vivo and a substrate for the Mps1p kinase in vitro. Conditional alleles of MOB1 cause a late nuclear division arrest at restrictive temperature. MOB1 exhibits genetic interaction with three other yeast genes required for the completion of mitosis, LTE1, CDC5, and CDC15 (the latter two encode essential protein kinases). Most haploid mutant mob1 strains also display a complete increase in ploidy at permissive temperature. The mechanism for the increase in ploidy may occur through MPS1 function. One mob1 strain, which maintains stable haploidy at both permissive and restrictive temperature, diploidizes at permissive temperature when combined with the mps1-1 mutation. Strains containing mob2Δ also display a complete increase in ploidy when combined with the mps1-1 mutation. Perhaps in addition to, or as part of, its essential function in late mitosis, MOB1 is required for a cell cycle reset function necessary for the initiation of the spindle pole body duplication.",
author = "Luca, {Francis C.} and Mark Winey",
year = "1998",
month = "1",
day = "1",
doi = "10.1091/mbc.9.1.29",
language = "English (US)",
volume = "9",
pages = "29--46",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "1",

}

TY - JOUR

T1 - Mob1, an essential yeast gene required for completion of mitosis and maintenance of ploidy

AU - Luca, Francis C.

AU - Winey, Mark

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Mob1p is an essential Saccharomyces cerevisiae protein, identified from a two-hybrid screen, that binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob1p contains no known structural motifs; however MOB1 is a member of a conserved gene family and shares sequence similarity with a nonessential yeast gene, MOB2. Mob1p is a phosphoprotein in vivo and a substrate for the Mps1p kinase in vitro. Conditional alleles of MOB1 cause a late nuclear division arrest at restrictive temperature. MOB1 exhibits genetic interaction with three other yeast genes required for the completion of mitosis, LTE1, CDC5, and CDC15 (the latter two encode essential protein kinases). Most haploid mutant mob1 strains also display a complete increase in ploidy at permissive temperature. The mechanism for the increase in ploidy may occur through MPS1 function. One mob1 strain, which maintains stable haploidy at both permissive and restrictive temperature, diploidizes at permissive temperature when combined with the mps1-1 mutation. Strains containing mob2Δ also display a complete increase in ploidy when combined with the mps1-1 mutation. Perhaps in addition to, or as part of, its essential function in late mitosis, MOB1 is required for a cell cycle reset function necessary for the initiation of the spindle pole body duplication.

AB - Mob1p is an essential Saccharomyces cerevisiae protein, identified from a two-hybrid screen, that binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob1p contains no known structural motifs; however MOB1 is a member of a conserved gene family and shares sequence similarity with a nonessential yeast gene, MOB2. Mob1p is a phosphoprotein in vivo and a substrate for the Mps1p kinase in vitro. Conditional alleles of MOB1 cause a late nuclear division arrest at restrictive temperature. MOB1 exhibits genetic interaction with three other yeast genes required for the completion of mitosis, LTE1, CDC5, and CDC15 (the latter two encode essential protein kinases). Most haploid mutant mob1 strains also display a complete increase in ploidy at permissive temperature. The mechanism for the increase in ploidy may occur through MPS1 function. One mob1 strain, which maintains stable haploidy at both permissive and restrictive temperature, diploidizes at permissive temperature when combined with the mps1-1 mutation. Strains containing mob2Δ also display a complete increase in ploidy when combined with the mps1-1 mutation. Perhaps in addition to, or as part of, its essential function in late mitosis, MOB1 is required for a cell cycle reset function necessary for the initiation of the spindle pole body duplication.

UR - http://www.scopus.com/inward/record.url?scp=0031909440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031909440&partnerID=8YFLogxK

U2 - 10.1091/mbc.9.1.29

DO - 10.1091/mbc.9.1.29

M3 - Article

C2 - 9436989

AN - SCOPUS:0031909440

VL - 9

SP - 29

EP - 46

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 1

ER -