Mixed infections of Mycobacterium tuberculosis in tuberculosis patients in Shanghai, China

Rendong Fang, Xia Li, Jing Li, Jie Wu, Xin Shen, Xiaohong Gui, Kathryn DeRiemer, Li Liu, Jian Mei, Qian Gao

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Abstract

We applied a 7 loci Variable-Number-Tandem-Repeats (VNTR-7) analysis method to identify mixed infections of Mycobacterium tuberculosis and to estimate the rate of mixed infections among pulmonary tuberculosis patients in Shanghai, China. We validated the VNTR-7 method and used it to genotype an isolate from each of the 249 from pulmonary tuberculosis patients reported from the Songjiang and Chongming districts in Shanghai during 2006. We identified 14 patients with mixed infections, and the estimated rate of mixed infections was 5.6% (14/249) (95% CI 3.1%-9.2%). Mixed infections were observed more frequently among tuberculosis patients undergoing retreatment (15.6%) than among new cases (4.1%) (p < 0.05), and among tuberculosis patients whose disease was caused by non-Beijing genotype strains (12.5%) versus Beijing genotype strains (3.5%) (p < 0.05). The VNTR-7 method is a highly sensitive, practical tool with relatively high discriminatory power, making it useful for studying mixed infections.

Original languageEnglish (US)
Pages (from-to)469-473
Number of pages5
JournalTuberculosis
Volume88
Issue number5
DOIs
StatePublished - Sep 2008

Fingerprint

Mycobacterium tuberculosis
Coinfection
mixed infection
tuberculosis
China
Tuberculosis
minisatellite repeats
Genotype
Pulmonary Tuberculosis
genotype
lungs
Minisatellite Repeats
Retreatment
methodology
loci

Keywords

  • Mixed infections
  • Tuberculosis
  • VNTR

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Immunology and Allergy
  • Infectious Diseases
  • veterinary(all)

Cite this

Mixed infections of Mycobacterium tuberculosis in tuberculosis patients in Shanghai, China. / Fang, Rendong; Li, Xia; Li, Jing; Wu, Jie; Shen, Xin; Gui, Xiaohong; DeRiemer, Kathryn; Liu, Li; Mei, Jian; Gao, Qian.

In: Tuberculosis, Vol. 88, No. 5, 09.2008, p. 469-473.

Research output: Contribution to journalArticle

Fang, R, Li, X, Li, J, Wu, J, Shen, X, Gui, X, DeRiemer, K, Liu, L, Mei, J & Gao, Q 2008, 'Mixed infections of Mycobacterium tuberculosis in tuberculosis patients in Shanghai, China', Tuberculosis, vol. 88, no. 5, pp. 469-473. https://doi.org/10.1016/j.tube.2008.02.002
Fang, Rendong ; Li, Xia ; Li, Jing ; Wu, Jie ; Shen, Xin ; Gui, Xiaohong ; DeRiemer, Kathryn ; Liu, Li ; Mei, Jian ; Gao, Qian. / Mixed infections of Mycobacterium tuberculosis in tuberculosis patients in Shanghai, China. In: Tuberculosis. 2008 ; Vol. 88, No. 5. pp. 469-473.
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