Mitotic spindle form and function

Mark Winey, Kerry Bloom

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The Saccharomyces cerevisiae mitotic spindle in budding yeast is exemplified by its simplicity and elegance. Microtubules are nucleated from a crystalline array of proteins organized in the nuclear envelope, known as the spindle pole body in yeast (analogous to the centrosome in larger eukaryotes). The spindle has two classes of nuclear microtubules: kinetochore microtubules and interpolar microtubules. One kinetochore microtubule attaches to a single centromere on each chromosome, while approximately four interpolar microtubules emanate from each pole and interdigitate with interpolar microtubules from the opposite spindle to provide stability to the bipolar spindle. On the cytoplasmic face, two to three microtubules extend from the spindle pole toward the cell cortex. Processes requiring microtubule function are limited to spindles in mitosis and to spindle orientation and nuclear positioning in the cytoplasm. Microtubule function is regulated in large part via products of the 6 kinesin gene family and the 1 cytoplasmic dynein gene. A single bipolar kinesin (Cin8, class Kin-5), together with a depolymerase (Kip3, class Kin-8) or minus-end-directed kinesin (Kar3, class Kin-14), can support spindle function and cell viability. The remarkable feature of yeast cells is that they can survive with microtubules and genes for just two motor proteins, thus providing an unparalleled system to dissect microtubule and motor function within the spindle machine.

Original languageEnglish (US)
Pages (from-to)1197-1224
Number of pages28
JournalGenetics
Volume190
Issue number4
DOIs
StatePublished - Apr 1 2012
Externally publishedYes

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Spindle Apparatus
Microtubules
Kinesin
Kinetochores
Yeasts
Spindle Pole Bodies
Cytoplasmic Dyneins
Spindle Poles
Genes
Protein Array Analysis
Centrosome
Saccharomycetales
Centromere
Nuclear Envelope
Eukaryota
Mitosis
Saccharomyces cerevisiae
Cell Survival
Cytoplasm
Chromosomes

ASJC Scopus subject areas

  • Genetics

Cite this

Mitotic spindle form and function. / Winey, Mark; Bloom, Kerry.

In: Genetics, Vol. 190, No. 4, 01.04.2012, p. 1197-1224.

Research output: Contribution to journalArticle

Winey, Mark ; Bloom, Kerry. / Mitotic spindle form and function. In: Genetics. 2012 ; Vol. 190, No. 4. pp. 1197-1224.
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