Mitogen-activated protein kinase regulates early phosphorylation and delayed expression of Ca2+/calmodulin-dependent protein kinase II in long-term potentiation

Maria Grazia Giovannini, Robert D. Blitzer, Tony Wong, Kichiemon Asoma, Panayiotis Tsokas, John Morrison, Ravi Iyengar, Emmanuel M. Landau

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Activation of mitogen-activated protein kinase (MAPK) and Ca2+/calmodulin protein kinase II (CaMKII) are required for numerous forms of neuronal plasticity, including long-term potentiation (LTP). We induced LTP in rat hippocampal area CA1 using theta-pulse stimulation (TPS) paired with β-adrenergic receptor activation [isoproterenol (ISO)], a protocol that may be particularly relevant to normal patterns of hippocampal activity during learning. This stimulation resulted in a transient phosphorylation of p42 MAPK, and the resulting LTP was MAPK dependent. In addition, CaMKII was regulated in two, temporally distinct ways after TPS-ISO: a transient rise in the fraction of phosphorylated CaMKII and a subsequent persistent increase in CaMKII expression. The increases in MAPK and CaMKII phosphorylation were strongly colocalized in the dendrites and cell bodies of CA1 pyramidal cells, and both the transient phosphorylation and delayed expression of CaMKII were prevented by inhibiting p42/p44 MAPK. These results establish a novel bimodal regulation of CaMKII by MAPK, which may contribute to both post-translational modification and increased gene expression.

Original languageEnglish (US)
Pages (from-to)7053-7062
Number of pages10
JournalJournal of Neuroscience
Volume21
Issue number18
StatePublished - Sep 15 2001
Externally publishedYes

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases
Long-Term Potentiation
Mitogen-Activated Protein Kinases
Protein Kinases
Phosphorylation
Mitogen-Activated Protein Kinase 1
Isoproterenol
Neuronal Plasticity
Pyramidal Cells
Post Translational Protein Processing
Dendrites
Adrenergic Receptors
Learning
Gene Expression

Keywords

  • β-adrenergic receptors
  • Ca/calmodulin-dependent protein kinase II
  • Erk
  • Long-term potentiation
  • Mitogen-activated protein kinase
  • Phosphorylation
  • Protein synthesis
  • Rat hippocampus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mitogen-activated protein kinase regulates early phosphorylation and delayed expression of Ca2+/calmodulin-dependent protein kinase II in long-term potentiation. / Giovannini, Maria Grazia; Blitzer, Robert D.; Wong, Tony; Asoma, Kichiemon; Tsokas, Panayiotis; Morrison, John; Iyengar, Ravi; Landau, Emmanuel M.

In: Journal of Neuroscience, Vol. 21, No. 18, 15.09.2001, p. 7053-7062.

Research output: Contribution to journalArticle

Giovannini, Maria Grazia ; Blitzer, Robert D. ; Wong, Tony ; Asoma, Kichiemon ; Tsokas, Panayiotis ; Morrison, John ; Iyengar, Ravi ; Landau, Emmanuel M. / Mitogen-activated protein kinase regulates early phosphorylation and delayed expression of Ca2+/calmodulin-dependent protein kinase II in long-term potentiation. In: Journal of Neuroscience. 2001 ; Vol. 21, No. 18. pp. 7053-7062.
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