Mitochondrial ultrastructure and markers of dynamics in hepatocytes from aged, calorie restricted mice fed with different dietary fats

Husam Khraiwesh, José A. López-Domínguez, Lucía Fernández del Río, Elena Gutierrez-Casado, Guillermo López-Lluch, Plácido Navas, Rafael de Cabo, Jon J Ramsey, María I. Burón, José M. Villalba, José A. González-Reyes

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

In this paper we analyzed changes in hepatocyte mitochondrial mass and ultrastructure as well as in mitochondrial markers of fission/fusion and biogenesis in mice subjected to 40% calorie restriction (CR) for 18. months versus ad libitum-fed controls. Animals subjected to CR were separated into three groups with different dietary fats: soybean oil (also in controls), fish oil and lard. Therefore, the effect of the dietary fat under CR was studied as well. Our results show that CR induced changes in hepatocyte and mitochondrial size, in the volume fraction occupied by mitochondria, and in the number of mitochondria per hepatocyte. Also, mean number of mitochondrial cristae and lengths were significantly higher in all CR groups compared with controls. Finally, CR had no remarkable effects on the expression levels of fission and fusion protein markers. However, considerable differences in many of these parameters were found when comparing the CR groups, supporting the idea that dietary fat plays a relevant role in the modulation of CR effects in aged mice.

Original languageEnglish (US)
Pages (from-to)77-88
Number of pages12
JournalExperimental Gerontology
Volume56
DOIs
StatePublished - 2014

Keywords

  • Calorie restriction
  • Dietary fat
  • Hepatocyte
  • Mature/old-aged mice
  • Mitochondria

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Genetics
  • Molecular Biology
  • Medicine(all)

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  • Cite this

    Khraiwesh, H., López-Domínguez, J. A., Fernández del Río, L., Gutierrez-Casado, E., López-Lluch, G., Navas, P., de Cabo, R., Ramsey, J. J., Burón, M. I., Villalba, J. M., & González-Reyes, J. A. (2014). Mitochondrial ultrastructure and markers of dynamics in hepatocytes from aged, calorie restricted mice fed with different dietary fats. Experimental Gerontology, 56, 77-88. https://doi.org/10.1016/j.exger.2014.03.023