Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media

Christa R. Darr, Gino A Cortopassi, Sandipan Datta, Dickson D. Varner, Stuart A Meyers

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freezin–“LE” and “MFR5”, in addition to several other parameters including motility, reactive oxygen species (ROS) production and viability. All experimental endpoints, with the exception of average path velocity, were affected significantly by freezing extender type after freezing and thawing. Sperm in INRA 96 had the lowest average progressive motility after thawing (24 ± 4.8%, P < 0.05). Sperm in EZ Freezin–“LE” had the highest post thaw viability (79 ± 3.1%, P < 0.05) and lowest post thaw ROS production (13 ± 2.4%), but sperm in BotuCrio had the highest maximal oxygen consumption levels, while also demonstrating similar ROS production and viability. This difference would not have been detected using conventional sperm analytical methods. In addition, sperm in BotuCrio had the highest average total motility (49 ± 7.4%), progressive motility (41 ± 6.4%), and velocity (VAP, 90 ± 3.6 μm/s) indicating that this medium preserved mitochondrial function optimally after cryopreservation. Mitochondrial oxygen consumption was positively correlated with traditional measures of sperm function including motility and viability (r = 0.62 and r = 0.49, respectively, P < 0.05), thus making it a sensitive method for determining cryopreservation success and mitochondrial function in stallion sperm.

Original languageEnglish (US)
Pages (from-to)1382-1392
Number of pages11
JournalTheriogenology
Volume86
Issue number5
DOIs
StatePublished - Sep 15 2016

Fingerprint

Cryopreservation
stallions
Oxygen Consumption
cryopreservation
oxygen consumption
Spermatozoa
spermatozoa
Health
viability
reactive oxygen species
Reactive Oxygen Species
thawing
Freezing
freezing
endpoints
Horses
analytical methods
Mitochondria
mitochondria
horses

Keywords

  • Cryopreservation extender
  • Mitochondria
  • Sperm
  • Stallion

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media. / Darr, Christa R.; Cortopassi, Gino A; Datta, Sandipan; Varner, Dickson D.; Meyers, Stuart A.

In: Theriogenology, Vol. 86, No. 5, 15.09.2016, p. 1382-1392.

Research output: Contribution to journalArticle

@article{335eefaa640846a3b38ee6647fd1ea0e,
title = "Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media",
abstract = "Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freezin–“LE” and “MFR5”, in addition to several other parameters including motility, reactive oxygen species (ROS) production and viability. All experimental endpoints, with the exception of average path velocity, were affected significantly by freezing extender type after freezing and thawing. Sperm in INRA 96 had the lowest average progressive motility after thawing (24 ± 4.8{\%}, P < 0.05). Sperm in EZ Freezin–“LE” had the highest post thaw viability (79 ± 3.1{\%}, P < 0.05) and lowest post thaw ROS production (13 ± 2.4{\%}), but sperm in BotuCrio had the highest maximal oxygen consumption levels, while also demonstrating similar ROS production and viability. This difference would not have been detected using conventional sperm analytical methods. In addition, sperm in BotuCrio had the highest average total motility (49 ± 7.4{\%}), progressive motility (41 ± 6.4{\%}), and velocity (VAP, 90 ± 3.6 μm/s) indicating that this medium preserved mitochondrial function optimally after cryopreservation. Mitochondrial oxygen consumption was positively correlated with traditional measures of sperm function including motility and viability (r = 0.62 and r = 0.49, respectively, P < 0.05), thus making it a sensitive method for determining cryopreservation success and mitochondrial function in stallion sperm.",
keywords = "Cryopreservation extender, Mitochondria, Sperm, Stallion",
author = "Darr, {Christa R.} and Cortopassi, {Gino A} and Sandipan Datta and Varner, {Dickson D.} and Meyers, {Stuart A}",
year = "2016",
month = "9",
day = "15",
doi = "10.1016/j.theriogenology.2016.04.082",
language = "English (US)",
volume = "86",
pages = "1382--1392",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media

AU - Darr, Christa R.

AU - Cortopassi, Gino A

AU - Datta, Sandipan

AU - Varner, Dickson D.

AU - Meyers, Stuart A

PY - 2016/9/15

Y1 - 2016/9/15

N2 - Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freezin–“LE” and “MFR5”, in addition to several other parameters including motility, reactive oxygen species (ROS) production and viability. All experimental endpoints, with the exception of average path velocity, were affected significantly by freezing extender type after freezing and thawing. Sperm in INRA 96 had the lowest average progressive motility after thawing (24 ± 4.8%, P < 0.05). Sperm in EZ Freezin–“LE” had the highest post thaw viability (79 ± 3.1%, P < 0.05) and lowest post thaw ROS production (13 ± 2.4%), but sperm in BotuCrio had the highest maximal oxygen consumption levels, while also demonstrating similar ROS production and viability. This difference would not have been detected using conventional sperm analytical methods. In addition, sperm in BotuCrio had the highest average total motility (49 ± 7.4%), progressive motility (41 ± 6.4%), and velocity (VAP, 90 ± 3.6 μm/s) indicating that this medium preserved mitochondrial function optimally after cryopreservation. Mitochondrial oxygen consumption was positively correlated with traditional measures of sperm function including motility and viability (r = 0.62 and r = 0.49, respectively, P < 0.05), thus making it a sensitive method for determining cryopreservation success and mitochondrial function in stallion sperm.

AB - Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freezin–“LE” and “MFR5”, in addition to several other parameters including motility, reactive oxygen species (ROS) production and viability. All experimental endpoints, with the exception of average path velocity, were affected significantly by freezing extender type after freezing and thawing. Sperm in INRA 96 had the lowest average progressive motility after thawing (24 ± 4.8%, P < 0.05). Sperm in EZ Freezin–“LE” had the highest post thaw viability (79 ± 3.1%, P < 0.05) and lowest post thaw ROS production (13 ± 2.4%), but sperm in BotuCrio had the highest maximal oxygen consumption levels, while also demonstrating similar ROS production and viability. This difference would not have been detected using conventional sperm analytical methods. In addition, sperm in BotuCrio had the highest average total motility (49 ± 7.4%), progressive motility (41 ± 6.4%), and velocity (VAP, 90 ± 3.6 μm/s) indicating that this medium preserved mitochondrial function optimally after cryopreservation. Mitochondrial oxygen consumption was positively correlated with traditional measures of sperm function including motility and viability (r = 0.62 and r = 0.49, respectively, P < 0.05), thus making it a sensitive method for determining cryopreservation success and mitochondrial function in stallion sperm.

KW - Cryopreservation extender

KW - Mitochondria

KW - Sperm

KW - Stallion

UR - http://www.scopus.com/inward/record.url?scp=84970024744&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84970024744&partnerID=8YFLogxK

U2 - 10.1016/j.theriogenology.2016.04.082

DO - 10.1016/j.theriogenology.2016.04.082

M3 - Article

C2 - 27242178

AN - SCOPUS:84970024744

VL - 86

SP - 1382

EP - 1392

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 5

ER -