Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media

Christa R. Darr, Gino A Cortopassi, Sandipan Datta, Dickson D. Varner, Stuart A Meyers

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freezin–“LE” and “MFR5”, in addition to several other parameters including motility, reactive oxygen species (ROS) production and viability. All experimental endpoints, with the exception of average path velocity, were affected significantly by freezing extender type after freezing and thawing. Sperm in INRA 96 had the lowest average progressive motility after thawing (24 ± 4.8%, P < 0.05). Sperm in EZ Freezin–“LE” had the highest post thaw viability (79 ± 3.1%, P < 0.05) and lowest post thaw ROS production (13 ± 2.4%), but sperm in BotuCrio had the highest maximal oxygen consumption levels, while also demonstrating similar ROS production and viability. This difference would not have been detected using conventional sperm analytical methods. In addition, sperm in BotuCrio had the highest average total motility (49 ± 7.4%), progressive motility (41 ± 6.4%), and velocity (VAP, 90 ± 3.6 μm/s) indicating that this medium preserved mitochondrial function optimally after cryopreservation. Mitochondrial oxygen consumption was positively correlated with traditional measures of sperm function including motility and viability (r = 0.62 and r = 0.49, respectively, P < 0.05), thus making it a sensitive method for determining cryopreservation success and mitochondrial function in stallion sperm.

Original languageEnglish (US)
Pages (from-to)1382-1392
Number of pages11
Issue number5
StatePublished - Sep 15 2016


  • Cryopreservation extender
  • Mitochondria
  • Sperm
  • Stallion

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine


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