Mitochondrial oxidant stress increases PDE5 activity in persistent pulmonary hypertension of the newborn

Kathryn N. Farrow, Stephen Wedgwood, Keng Jin Lee, Lyubov Czech, Sylvia F. Gugino, Satyanarayana Lakshminrusimha, Paul T. Schumacker, Robin H Steinhorn

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

In the pulmonary vasculature, phosphodiesterase-5 (PDE5) degrades cGMP and inhibits nitric oxide-mediated, cGMP-dependent vasorelaxation. We previously reported that ventilation with 100% O2 increased PDE5 activity in pulmonary arteries (PAs) of pulmonary hypertension lambs (PPHN) more than in control lambs. In the present study, PA smooth muscle cells (PASMCs) from PPHN lambs had increased basal PDE5 activity, decreased cGMP-responsiveness to NO, and increased mitochondrial matrix oxidant stress compared to control PASMC. Hyperoxia (24h) increased PDE5 activity and mitochondrial matrix oxidant stress above baseline to a similar degree in PPHN and control PASMC. Mitochondrially targeted catalase decreased PDE5 activity at baseline and after hyperoxia in PPHN PASMC. Similarly, catalase treatment of PPHN lambs ventilated with 100% O2 decreased PDE5 activity and increased cGMP in PA. We conclude that baseline PDE5 activity and oxidative stress is increased in PPHN PASMC, and scavenging H2O2 is sufficient to block oxidant-mediated increases in PDE5 activity in PPHN.

Original languageEnglish (US)
Pages (from-to)272-281
Number of pages10
JournalRespiratory Physiology and Neurobiology
Volume174
Issue number3
DOIs
StatePublished - Dec 31 2010
Externally publishedYes

Keywords

  • CGMP
  • Hyperoxia
  • Phosphodiesterase
  • Pulmonary vasculature
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Neuroscience(all)

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