Mitochondrial nucleic acids as internal standards for blot hybridization analyses

Clifford G Tepper, Mary M. Pater, Alan Pater, Hui min Xu, George P. Studzinski

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A plasmid, designated p72, constructed from human lung carcinoma DNA inserted into the promoterless herpes simplex virus thymidine kinase gene pML-TK-Bgl II vector, hybridizes strongly to human nucleic acids on Southern and Northern blots. The portion of the DNA insert responsible for the strong signal following hybridization to human DNA or RNA is a 167-bp 3′ terminal portion of the mitochondrial 16S ribosomal RNA gene. The expression of this gene is constitutive in the several human cell lines that were tested and is unaffected by exposure to cytotoxic chemicals that alter the expression of nuclear genes. This plasmid offers an excellent tool for studies of perturbations of gene expression and for controlling for the variations in sample preparation, loading, and transfer in Southern or Northern analysis of nucleic acids.

Original languageEnglish (US)
Pages (from-to)127-133
Number of pages7
JournalAnalytical Biochemistry
Volume203
Issue number1
DOIs
StatePublished - May 15 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Tepper, C. G., Pater, M. M., Pater, A., Xu, H. M., & Studzinski, G. P. (1992). Mitochondrial nucleic acids as internal standards for blot hybridization analyses. Analytical Biochemistry, 203(1), 127-133. https://doi.org/10.1016/0003-2697(92)90052-9