Mitochondrial Inner-Membrane Fusion and Crista Maintenance Requires the Dynamin-Related GTPase Mgm1

Shelly Meeusen, Rachel DeVay, Jennifer Block, Ann Cassidy-Stone, Sarah Wayson, J. Michael McCaffery, Jodi Nunnari

Research output: Contribution to journalArticle

277 Citations (Scopus)

Abstract

Mitochondrial outer- and inner-membrane fusion events are coupled in vivo but separable and mechanistically distinct in vitro, indicating that separate fusion machines exist in each membrane. Outer-membrane fusion requires trans interactions of the dynamin-related GTPase Fzo1, GTP hydrolysis, and an intact inner-membrane proton gradient. Inner-membrane fusion also requires GTP hydrolysis but distinctly requires an inner-membrane electrical potential. The protein machinery responsible for inner-membrane fusion is unknown. Here, we show that the conserved intermembrane-space dynamin-related GTPase Mgm1 is required to tether and fuse mitochondrial inner membranes. We observe an additional role of Mgm1 in inner-membrane dynamics, specifically in the maintenance of crista structures. We present evidence that trans Mgm1 interactions on opposing inner membranes function similarly to tether and fuse inner membranes as well as maintain crista structures and propose a model for how the mitochondrial dynamins function to facilitate fusion.

Original languageEnglish (US)
Pages (from-to)383-395
Number of pages13
JournalCell
Volume127
Issue number2
DOIs
StatePublished - Oct 20 2006

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Membrane Fusion
GTP Phosphohydrolases
Dynamins
Fusion reactions
Maintenance
Membranes
Guanosine Triphosphate
Hydrolysis
Mitochondrial Membranes
Electric fuses
Membrane Potentials
Protons
Machinery
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Meeusen, S., DeVay, R., Block, J., Cassidy-Stone, A., Wayson, S., McCaffery, J. M., & Nunnari, J. (2006). Mitochondrial Inner-Membrane Fusion and Crista Maintenance Requires the Dynamin-Related GTPase Mgm1. Cell, 127(2), 383-395. https://doi.org/10.1016/j.cell.2006.09.021

Mitochondrial Inner-Membrane Fusion and Crista Maintenance Requires the Dynamin-Related GTPase Mgm1. / Meeusen, Shelly; DeVay, Rachel; Block, Jennifer; Cassidy-Stone, Ann; Wayson, Sarah; McCaffery, J. Michael; Nunnari, Jodi.

In: Cell, Vol. 127, No. 2, 20.10.2006, p. 383-395.

Research output: Contribution to journalArticle

Meeusen, S, DeVay, R, Block, J, Cassidy-Stone, A, Wayson, S, McCaffery, JM & Nunnari, J 2006, 'Mitochondrial Inner-Membrane Fusion and Crista Maintenance Requires the Dynamin-Related GTPase Mgm1', Cell, vol. 127, no. 2, pp. 383-395. https://doi.org/10.1016/j.cell.2006.09.021
Meeusen S, DeVay R, Block J, Cassidy-Stone A, Wayson S, McCaffery JM et al. Mitochondrial Inner-Membrane Fusion and Crista Maintenance Requires the Dynamin-Related GTPase Mgm1. Cell. 2006 Oct 20;127(2):383-395. https://doi.org/10.1016/j.cell.2006.09.021
Meeusen, Shelly ; DeVay, Rachel ; Block, Jennifer ; Cassidy-Stone, Ann ; Wayson, Sarah ; McCaffery, J. Michael ; Nunnari, Jodi. / Mitochondrial Inner-Membrane Fusion and Crista Maintenance Requires the Dynamin-Related GTPase Mgm1. In: Cell. 2006 ; Vol. 127, No. 2. pp. 383-395.
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