Mitochondrial genomes of Anopheles arabiensis, An. gambiae and An. coluzzii show no clear species division

Mark J. Hanemaaijer, Parker D. Houston, Travis C. Collier, Laura C. Norris, Abdrahamane Fofana, Gregory C Lanzaro, Anthony J. Cornel, Yoosook Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Here we report the complete mitochondrial sequences of 70 individual field collected mosquito specimens from throughout Sub-Saharan Africa. We generated this dataset to identify species specific markers for the following Anopheles species and chromosomal forms: An. arabiensis, An. coluzzii (The Forest and Mopti chromosomal forms) and An. gambiae (The Bamako and Savannah chromosomal forms).  The raw Illumina sequencing reads were mapped to the NC_002084 reference mitogenome sequence. A total of 783 single nucleotide polymorphisms (SNPs) were detected on the mitochondrial genome, of which 460 are singletons (58.7%). None of these SNPs are suitable as molecular markers to distinguish among An. arabiensis, An. coluzzii and An. gambiae or any of the chromosomal forms. The lack of species or chromosomal form specific markers is also reflected in the constructed phylogenetic tree, which shows no clear division among the operational taxonomic units considered here.

Original languageEnglish (US)
JournalF1000Research
Volume7
DOIs
StatePublished - Mar 15 2019

Fingerprint

Anopheles
Mitochondrial Genome
Polymorphism
Single Nucleotide Polymorphism
Nucleotides
Genes
Africa South of the Sahara
Culicidae
Datasets
Forests

Keywords

  • Africa
  • Anopheles
  • Malaria vector
  • Mitogenome
  • Mosquitoes
  • Phylogenomics
  • Single nucleotide polymorphisms
  • Species identification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Mitochondrial genomes of Anopheles arabiensis, An. gambiae and An. coluzzii show no clear species division. / Hanemaaijer, Mark J.; Houston, Parker D.; Collier, Travis C.; Norris, Laura C.; Fofana, Abdrahamane; Lanzaro, Gregory C; Cornel, Anthony J.; Lee, Yoosook.

In: F1000Research, Vol. 7, 15.03.2019.

Research output: Contribution to journalArticle

Hanemaaijer, Mark J. ; Houston, Parker D. ; Collier, Travis C. ; Norris, Laura C. ; Fofana, Abdrahamane ; Lanzaro, Gregory C ; Cornel, Anthony J. ; Lee, Yoosook. / Mitochondrial genomes of Anopheles arabiensis, An. gambiae and An. coluzzii show no clear species division. In: F1000Research. 2019 ; Vol. 7.
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