Mitochondrial fusion intermediates revealed in vitro

Shelly Meeusen, J. Michael McCaffery, Jodi Nunnari

Research output: Contribution to journalArticle

307 Citations (Scopus)

Abstract

The events that occur during the fusion of double-membraned mitochondria are unknown. As an essential step toward determining the mechanism of mitochondrial fusion, we have captured this event in vitro. Mitochondrial outer and inner membrane fusion events were separable and mechanistically distinct, but both required guanosine 5′-triphosphate hydrolysis. Homotypic trans interactions of the ancient outer transmembrane guanosine triphosphatase, Fzol, were required to promote the fusion of mitochondrial outer membranes, whereas electrical potential was also required for fusion of inner membranes. Our conclusions provide fundamental insights into the molecular events driving mitochondrial fusion and advance our understanding of the evolution of mitochondrial fusion in eukaryotic cells.

Original languageEnglish (US)
Pages (from-to)1747-1752
Number of pages6
JournalScience
Volume305
Issue number5691
DOIs
StatePublished - Sep 17 2004

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Mitochondrial Dynamics
Membrane Fusion
Guanosine
Mitochondrial Membranes
Eukaryotic Cells
Guanosine Triphosphate
Mitochondria
Hydrolysis
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Meeusen, S., McCaffery, J. M., & Nunnari, J. (2004). Mitochondrial fusion intermediates revealed in vitro. Science, 305(5691), 1747-1752. https://doi.org/10.1126/science.1100612

Mitochondrial fusion intermediates revealed in vitro. / Meeusen, Shelly; McCaffery, J. Michael; Nunnari, Jodi.

In: Science, Vol. 305, No. 5691, 17.09.2004, p. 1747-1752.

Research output: Contribution to journalArticle

Meeusen, S, McCaffery, JM & Nunnari, J 2004, 'Mitochondrial fusion intermediates revealed in vitro', Science, vol. 305, no. 5691, pp. 1747-1752. https://doi.org/10.1126/science.1100612
Meeusen S, McCaffery JM, Nunnari J. Mitochondrial fusion intermediates revealed in vitro. Science. 2004 Sep 17;305(5691):1747-1752. https://doi.org/10.1126/science.1100612
Meeusen, Shelly ; McCaffery, J. Michael ; Nunnari, Jodi. / Mitochondrial fusion intermediates revealed in vitro. In: Science. 2004 ; Vol. 305, No. 5691. pp. 1747-1752.
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