Mitochondrial fusion intermediates revealed in vitro

Shelly Meeusen, J. Michael McCaffery, Jodi Nunnari

Research output: Contribution to journalArticlepeer-review

324 Scopus citations

Abstract

The events that occur during the fusion of double-membraned mitochondria are unknown. As an essential step toward determining the mechanism of mitochondrial fusion, we have captured this event in vitro. Mitochondrial outer and inner membrane fusion events were separable and mechanistically distinct, but both required guanosine 5′-triphosphate hydrolysis. Homotypic trans interactions of the ancient outer transmembrane guanosine triphosphatase, Fzol, were required to promote the fusion of mitochondrial outer membranes, whereas electrical potential was also required for fusion of inner membranes. Our conclusions provide fundamental insights into the molecular events driving mitochondrial fusion and advance our understanding of the evolution of mitochondrial fusion in eukaryotic cells.

Original languageEnglish (US)
Pages (from-to)1747-1752
Number of pages6
JournalScience
Volume305
Issue number5691
DOIs
StatePublished - Sep 17 2004

ASJC Scopus subject areas

  • General

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