Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p

Greg J. Hermann, John W. Thatcher, John P. Mills, Karen G. Hales, Margaret T. Fuller, Jodi Nunnari, Janet M. Shaw

Research output: Contribution to journalArticle

386 Citations (Scopus)

Abstract

Membrane fusion is required to establish the morphology and cellular distribution of the mitochondrial compartment. In Drosophila, mutations in the fuzzy onions (fzo) GTPase block a developmentally regulated mitochondrial fusion event during spermatogenesis. Here we report that the yeast orthologue of fuzzy onions, Fzo1p, plays a direct and conserved role in mitochondrial fusion. A conditional fzo1 mutation causes the mitochondrial reticulum to fragment and blocks mitochondrial fusion during yeast mating. Fzo1p is a mitochondrial integral membrane protein with its GTPase domain exposed to the cytoplasm. Point mutations that alter conserved residues in the GTPase do- main do not affect Fzo1p localization but disrupt mitochondrial fusion. Suborganellar fractionation suggests that Fzo1p spans the outer and is tightly associated with the inner mitochondrial membrane. This topology may be required to coordinate the behavior of the two mitochondrial membranes during the fusion reaction. We propose that the fuzzy onions family of transmembrane GTPases act as molecular switches to regulate a key step in mitochondrial membrane docking and/or fusion.

Original languageEnglish (US)
Pages (from-to)359-373
Number of pages15
JournalJournal of Cell Biology
Volume143
Issue number2
DOIs
StatePublished - Oct 19 1998

Fingerprint

Mitochondrial Dynamics
GTP Phosphohydrolases
Mitochondrial Membranes
Yeasts
Onions
Membrane Fusion
Reticulum
Mutation
Spermatogenesis
Point Mutation
Drosophila
Membrane Proteins
Cytoplasm

Keywords

  • GTPase
  • Membrane fusion
  • Mitochondria
  • mtDNA
  • Organelle morphology

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hermann, G. J., Thatcher, J. W., Mills, J. P., Hales, K. G., Fuller, M. T., Nunnari, J., & Shaw, J. M. (1998). Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p. Journal of Cell Biology, 143(2), 359-373. https://doi.org/10.1083/jcb.143.2.359

Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p. / Hermann, Greg J.; Thatcher, John W.; Mills, John P.; Hales, Karen G.; Fuller, Margaret T.; Nunnari, Jodi; Shaw, Janet M.

In: Journal of Cell Biology, Vol. 143, No. 2, 19.10.1998, p. 359-373.

Research output: Contribution to journalArticle

Hermann, GJ, Thatcher, JW, Mills, JP, Hales, KG, Fuller, MT, Nunnari, J & Shaw, JM 1998, 'Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p', Journal of Cell Biology, vol. 143, no. 2, pp. 359-373. https://doi.org/10.1083/jcb.143.2.359
Hermann GJ, Thatcher JW, Mills JP, Hales KG, Fuller MT, Nunnari J et al. Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p. Journal of Cell Biology. 1998 Oct 19;143(2):359-373. https://doi.org/10.1083/jcb.143.2.359
Hermann, Greg J. ; Thatcher, John W. ; Mills, John P. ; Hales, Karen G. ; Fuller, Margaret T. ; Nunnari, Jodi ; Shaw, Janet M. / Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p. In: Journal of Cell Biology. 1998 ; Vol. 143, No. 2. pp. 359-373.
@article{2f693e13870b4452b0d03fdd4e23136f,
title = "Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p",
abstract = "Membrane fusion is required to establish the morphology and cellular distribution of the mitochondrial compartment. In Drosophila, mutations in the fuzzy onions (fzo) GTPase block a developmentally regulated mitochondrial fusion event during spermatogenesis. Here we report that the yeast orthologue of fuzzy onions, Fzo1p, plays a direct and conserved role in mitochondrial fusion. A conditional fzo1 mutation causes the mitochondrial reticulum to fragment and blocks mitochondrial fusion during yeast mating. Fzo1p is a mitochondrial integral membrane protein with its GTPase domain exposed to the cytoplasm. Point mutations that alter conserved residues in the GTPase do- main do not affect Fzo1p localization but disrupt mitochondrial fusion. Suborganellar fractionation suggests that Fzo1p spans the outer and is tightly associated with the inner mitochondrial membrane. This topology may be required to coordinate the behavior of the two mitochondrial membranes during the fusion reaction. We propose that the fuzzy onions family of transmembrane GTPases act as molecular switches to regulate a key step in mitochondrial membrane docking and/or fusion.",
keywords = "GTPase, Membrane fusion, Mitochondria, mtDNA, Organelle morphology",
author = "Hermann, {Greg J.} and Thatcher, {John W.} and Mills, {John P.} and Hales, {Karen G.} and Fuller, {Margaret T.} and Jodi Nunnari and Shaw, {Janet M.}",
year = "1998",
month = "10",
day = "19",
doi = "10.1083/jcb.143.2.359",
language = "English (US)",
volume = "143",
pages = "359--373",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "2",

}

TY - JOUR

T1 - Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p

AU - Hermann, Greg J.

AU - Thatcher, John W.

AU - Mills, John P.

AU - Hales, Karen G.

AU - Fuller, Margaret T.

AU - Nunnari, Jodi

AU - Shaw, Janet M.

PY - 1998/10/19

Y1 - 1998/10/19

N2 - Membrane fusion is required to establish the morphology and cellular distribution of the mitochondrial compartment. In Drosophila, mutations in the fuzzy onions (fzo) GTPase block a developmentally regulated mitochondrial fusion event during spermatogenesis. Here we report that the yeast orthologue of fuzzy onions, Fzo1p, plays a direct and conserved role in mitochondrial fusion. A conditional fzo1 mutation causes the mitochondrial reticulum to fragment and blocks mitochondrial fusion during yeast mating. Fzo1p is a mitochondrial integral membrane protein with its GTPase domain exposed to the cytoplasm. Point mutations that alter conserved residues in the GTPase do- main do not affect Fzo1p localization but disrupt mitochondrial fusion. Suborganellar fractionation suggests that Fzo1p spans the outer and is tightly associated with the inner mitochondrial membrane. This topology may be required to coordinate the behavior of the two mitochondrial membranes during the fusion reaction. We propose that the fuzzy onions family of transmembrane GTPases act as molecular switches to regulate a key step in mitochondrial membrane docking and/or fusion.

AB - Membrane fusion is required to establish the morphology and cellular distribution of the mitochondrial compartment. In Drosophila, mutations in the fuzzy onions (fzo) GTPase block a developmentally regulated mitochondrial fusion event during spermatogenesis. Here we report that the yeast orthologue of fuzzy onions, Fzo1p, plays a direct and conserved role in mitochondrial fusion. A conditional fzo1 mutation causes the mitochondrial reticulum to fragment and blocks mitochondrial fusion during yeast mating. Fzo1p is a mitochondrial integral membrane protein with its GTPase domain exposed to the cytoplasm. Point mutations that alter conserved residues in the GTPase do- main do not affect Fzo1p localization but disrupt mitochondrial fusion. Suborganellar fractionation suggests that Fzo1p spans the outer and is tightly associated with the inner mitochondrial membrane. This topology may be required to coordinate the behavior of the two mitochondrial membranes during the fusion reaction. We propose that the fuzzy onions family of transmembrane GTPases act as molecular switches to regulate a key step in mitochondrial membrane docking and/or fusion.

KW - GTPase

KW - Membrane fusion

KW - Mitochondria

KW - mtDNA

KW - Organelle morphology

UR - http://www.scopus.com/inward/record.url?scp=0032547845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032547845&partnerID=8YFLogxK

U2 - 10.1083/jcb.143.2.359

DO - 10.1083/jcb.143.2.359

M3 - Article

VL - 143

SP - 359

EP - 373

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2

ER -