Mitochondrial dysfunction and calcium perturbation induced by traumatic brain injury

Y. Xiong, Q. Gu, P. L. Peterson, Jan Paul Muizelaar, C. P. Lee

Research output: Contribution to journalArticle

303 Citations (Scopus)

Abstract

Traumatic brain injury (TBI) is associated with primary and secondary injury. A thorough understanding of secondary injury will help to develop effective treatments and improve patient outcome. In this study, the GM model of controlled cortical impact injury (CCII) of Lighthall (1988) was used with modification to induce lateral TBI in rats. Forebrain mitochondria isolated from ipsilateral (IH) and contralateral (CH) hemispheres to impact showed a distinct difference. With glutamate + malate as substrates, mitochondria from the IH showed a significant decrease in State 3 respiratory rates, respiratory control indices (RCI), and P/O ratios. This decrease occurred as early as 1 h and persisted for at least 14 days following TBI. The State 3 respiratory rates, RCI, and P/O ratios could be restored to sham values by the addition of EGTA to the assay mixture. A significant amount of Ca2+ was found to be adsorbed to the mitochondria of both the IH and the CH with higher values seen in the IH. The rate of energy-linked Ca2+ transport in the IH was significantly decreased at 6 and 12 h. These data indicate that CCII-induced TBI perturbs cellular Ca2+ homeostasis and results in excessive Ca2+ adsorption to the mitochondrial membrane, which subsequently inhibits the respiratory chain-linked electron transfer and energy transduction.

Original languageEnglish (US)
Pages (from-to)23-34
Number of pages12
JournalJournal of Neurotrauma
Volume14
Issue number1
StatePublished - Jan 1997
Externally publishedYes

Fingerprint

Calcium
Mitochondria
Wounds and Injuries
Respiratory Rate
Egtazic Acid
Energy Transfer
Mitochondrial Membranes
Electron Transport
Prosencephalon
Adsorption
Glutamic Acid
Homeostasis
Electrons
Traumatic Brain Injury
Therapeutics
malic acid

Keywords

  • brain mitochondria
  • Ca
  • oxidative phosphorylation
  • rat
  • traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Mitochondrial dysfunction and calcium perturbation induced by traumatic brain injury. / Xiong, Y.; Gu, Q.; Peterson, P. L.; Muizelaar, Jan Paul; Lee, C. P.

In: Journal of Neurotrauma, Vol. 14, No. 1, 01.1997, p. 23-34.

Research output: Contribution to journalArticle

Xiong, Y. ; Gu, Q. ; Peterson, P. L. ; Muizelaar, Jan Paul ; Lee, C. P. / Mitochondrial dysfunction and calcium perturbation induced by traumatic brain injury. In: Journal of Neurotrauma. 1997 ; Vol. 14, No. 1. pp. 23-34.
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