Mitochondrial DNA Sequencing of Cat Hair: An Informative Forensic Tool

Christy R. Tarditi, Robert A Grahn, Jeffrey J. Evans, Jennifer D. Kurushima, Leslie A Lyons

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Approximately 81.7 million cats are in 37.5 million U.S. households. Shed fur can be criminal evidence because of transfer to victims, suspects, and/or their belongings. To improve cat hairs as forensic evidence, the mtDNA control region from single hairs, with and without root tags, was sequenced. A dataset of a 402-bp control region segment from 174 random-bred cats representing four U.S. geographic areas was generated to determine the informativeness of the mtDNA region. Thirty-two mtDNA mitotypes were observed ranging in frequencies from 0.6-27%. Four common types occurred in all populations. Low heteroplasmy, 1.7%, was determined. Unique mitotypes were found in 18 individuals, 10.3% of the population studied. The calculated discrimination power implied that 8.3 of 10 randomly selected individuals can be excluded by this region. The genetic characteristics of the region and the generated dataset support the use of this cat mtDNA region in forensic applications. 2010 American Academy of Forensic Sciences. Published 2010. This article is a U.S. Government work and is in the public domain in the U.S.A.

Original languageEnglish (US)
JournalJournal of Forensic Sciences
Issue numberSUPPL. 1
StatePublished - Jan 2011


  • Control region
  • DNA typing
  • Domestic cat
  • Felis catus
  • Forensic science
  • Mitochondrial DNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics


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