Mitochondrial DNA sequence heteroplasmy levels in domestic dog hair

Ashley M. Spicer; Teri J. Kun; Benjamin Sacks; Elizabeth J. Wictum

doi:10.1016/j.fsigen.2014.02.006

Mitochondrial DNA sequence heteroplasmy levels in domestic dog hair

Ashley M. Spicer, Teri J. Kun, Benjamin Sacks, Elizabeth J. Wictum

School of Veterinary Medicine

Research output: Contribution to journal › Article

2 Scopus citations

Abstract

To assess the level of mtDNA sequence heteroplasmy in dog hair, we sequenced a 612 base pair fragment of the hypervariable region 1 (HVI) in 576 hairs from six dogs representing a range of age, sex, breed, and hair color. Blood and buccal samples were collected from each dog for reference. Three instances of sequence heteroplasmy were observed at nucleotide positions 15627 (G/A), 15628 (T/C) and 15639 (G/A) in two hairs from different dogs. An HVI sequence heteroplasmy frequency of 0.0034 was obtained. The Probability of Identity (P_I) value, or probability that two random, unrelated dog hairs share an HVI sequence, and the Power of Discrimination (PD), or probability that two random unrelated dog hairs have different HVI sequences, were determined from the 88 HVI haplotypes represented in the Veterinary Genetics Laboratory database (n = 1006) and found to be 0.086 and 0.914, respectively.

Original language	English (US)
Pages (from-to)	7-12
Number of pages	6
Journal	Forensic Science International: Genetics
Volume	11
Issue number	1
DOIs	https://doi.org/10.1016/j.fsigen.2014.02.006
State	Published - Jan 1 2014

Fingerprint

Keywords

Canine
Dog
Forensic science
HVI
Mitchondrial
mtDNA
Sequence heteroplasmy

ASJC Scopus subject areas

Pathology and Forensic Medicine
Genetics
Medicine(all)

Cite this

Spicer, A. M., Kun, T. J., Sacks, B., & Wictum, E. J. (2014). Mitochondrial DNA sequence heteroplasmy levels in domestic dog hair. Forensic Science International: Genetics, 11(1), 7-12. https://doi.org/10.1016/j.fsigen.2014.02.006

@article{e0059adeb7604f00bd6f5ab50b512146,

title = "Mitochondrial DNA sequence heteroplasmy levels in domestic dog hair",

abstract = "To assess the level of mtDNA sequence heteroplasmy in dog hair, we sequenced a 612 base pair fragment of the hypervariable region 1 (HVI) in 576 hairs from six dogs representing a range of age, sex, breed, and hair color. Blood and buccal samples were collected from each dog for reference. Three instances of sequence heteroplasmy were observed at nucleotide positions 15627 (G/A), 15628 (T/C) and 15639 (G/A) in two hairs from different dogs. An HVI sequence heteroplasmy frequency of 0.0034 was obtained. The Probability of Identity (PI) value, or probability that two random, unrelated dog hairs share an HVI sequence, and the Power of Discrimination (PD), or probability that two random unrelated dog hairs have different HVI sequences, were determined from the 88 HVI haplotypes represented in the Veterinary Genetics Laboratory database (n = 1006) and found to be 0.086 and 0.914, respectively.",

keywords = "Canine, Dog, Forensic science, HVI, Mitchondrial, mtDNA, Sequence heteroplasmy",

author = "Spicer, {Ashley M.} and Kun, {Teri J.} and Benjamin Sacks and Wictum, {Elizabeth J.}",

year = "2014",

month = jan

day = "1",

doi = "10.1016/j.fsigen.2014.02.006",

language = "English (US)",

volume = "11",

pages = "7--12",

journal = "Forensic Science International: Genetics",

issn = "1872-4973",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Mitochondrial DNA sequence heteroplasmy levels in domestic dog hair

AU - Spicer, Ashley M.

AU - Kun, Teri J.

AU - Sacks, Benjamin

AU - Wictum, Elizabeth J.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - To assess the level of mtDNA sequence heteroplasmy in dog hair, we sequenced a 612 base pair fragment of the hypervariable region 1 (HVI) in 576 hairs from six dogs representing a range of age, sex, breed, and hair color. Blood and buccal samples were collected from each dog for reference. Three instances of sequence heteroplasmy were observed at nucleotide positions 15627 (G/A), 15628 (T/C) and 15639 (G/A) in two hairs from different dogs. An HVI sequence heteroplasmy frequency of 0.0034 was obtained. The Probability of Identity (PI) value, or probability that two random, unrelated dog hairs share an HVI sequence, and the Power of Discrimination (PD), or probability that two random unrelated dog hairs have different HVI sequences, were determined from the 88 HVI haplotypes represented in the Veterinary Genetics Laboratory database (n = 1006) and found to be 0.086 and 0.914, respectively.

AB - To assess the level of mtDNA sequence heteroplasmy in dog hair, we sequenced a 612 base pair fragment of the hypervariable region 1 (HVI) in 576 hairs from six dogs representing a range of age, sex, breed, and hair color. Blood and buccal samples were collected from each dog for reference. Three instances of sequence heteroplasmy were observed at nucleotide positions 15627 (G/A), 15628 (T/C) and 15639 (G/A) in two hairs from different dogs. An HVI sequence heteroplasmy frequency of 0.0034 was obtained. The Probability of Identity (PI) value, or probability that two random, unrelated dog hairs share an HVI sequence, and the Power of Discrimination (PD), or probability that two random unrelated dog hairs have different HVI sequences, were determined from the 88 HVI haplotypes represented in the Veterinary Genetics Laboratory database (n = 1006) and found to be 0.086 and 0.914, respectively.

KW - Canine

KW - Dog

KW - Forensic science

KW - HVI

KW - Mitchondrial

KW - mtDNA

KW - Sequence heteroplasmy

UR - http://www.scopus.com/inward/record.url?scp=84896532015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896532015&partnerID=8YFLogxK

U2 - 10.1016/j.fsigen.2014.02.006

DO - 10.1016/j.fsigen.2014.02.006

M3 - Article

C2 - 24631692

AN - SCOPUS:84896532015

VL - 11

SP - 7

EP - 12

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

SN - 1872-4973

IS - 1

ER -

Access to Document

10.1016/j.fsigen.2014.02.006