Abstract
To assess the level of mtDNA sequence heteroplasmy in dog hair, we sequenced a 612 base pair fragment of the hypervariable region 1 (HVI) in 576 hairs from six dogs representing a range of age, sex, breed, and hair color. Blood and buccal samples were collected from each dog for reference. Three instances of sequence heteroplasmy were observed at nucleotide positions 15627 (G/A), 15628 (T/C) and 15639 (G/A) in two hairs from different dogs. An HVI sequence heteroplasmy frequency of 0.0034 was obtained. The Probability of Identity (PI) value, or probability that two random, unrelated dog hairs share an HVI sequence, and the Power of Discrimination (PD), or probability that two random unrelated dog hairs have different HVI sequences, were determined from the 88 HVI haplotypes represented in the Veterinary Genetics Laboratory database (n = 1006) and found to be 0.086 and 0.914, respectively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7-12 |
| Number of pages | 6 |
| Journal | Forensic Science International: Genetics |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2014 |
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Keywords
- Canine
- Dog
- Forensic science
- HVI
- Mitchondrial
- mtDNA
- Sequence heteroplasmy
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Genetics
- Medicine(all)
Cite this
Mitochondrial DNA sequence heteroplasmy levels in domestic dog hair. / Spicer, Ashley M.; Kun, Teri J.; Sacks, Benjamin; Wictum, Elizabeth J.
In: Forensic Science International: Genetics, Vol. 11, No. 1, 01.01.2014, p. 7-12.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mitochondrial DNA sequence heteroplasmy levels in domestic dog hair
AU - Spicer, Ashley M.
AU - Kun, Teri J.
AU - Sacks, Benjamin
AU - Wictum, Elizabeth J.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - To assess the level of mtDNA sequence heteroplasmy in dog hair, we sequenced a 612 base pair fragment of the hypervariable region 1 (HVI) in 576 hairs from six dogs representing a range of age, sex, breed, and hair color. Blood and buccal samples were collected from each dog for reference. Three instances of sequence heteroplasmy were observed at nucleotide positions 15627 (G/A), 15628 (T/C) and 15639 (G/A) in two hairs from different dogs. An HVI sequence heteroplasmy frequency of 0.0034 was obtained. The Probability of Identity (PI) value, or probability that two random, unrelated dog hairs share an HVI sequence, and the Power of Discrimination (PD), or probability that two random unrelated dog hairs have different HVI sequences, were determined from the 88 HVI haplotypes represented in the Veterinary Genetics Laboratory database (n = 1006) and found to be 0.086 and 0.914, respectively.
AB - To assess the level of mtDNA sequence heteroplasmy in dog hair, we sequenced a 612 base pair fragment of the hypervariable region 1 (HVI) in 576 hairs from six dogs representing a range of age, sex, breed, and hair color. Blood and buccal samples were collected from each dog for reference. Three instances of sequence heteroplasmy were observed at nucleotide positions 15627 (G/A), 15628 (T/C) and 15639 (G/A) in two hairs from different dogs. An HVI sequence heteroplasmy frequency of 0.0034 was obtained. The Probability of Identity (PI) value, or probability that two random, unrelated dog hairs share an HVI sequence, and the Power of Discrimination (PD), or probability that two random unrelated dog hairs have different HVI sequences, were determined from the 88 HVI haplotypes represented in the Veterinary Genetics Laboratory database (n = 1006) and found to be 0.086 and 0.914, respectively.
KW - Canine
KW - Dog
KW - Forensic science
KW - HVI
KW - Mitchondrial
KW - mtDNA
KW - Sequence heteroplasmy
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UR - http://www.scopus.com/inward/citedby.url?scp=84896532015&partnerID=8YFLogxK
U2 - 10.1016/j.fsigen.2014.02.006
DO - 10.1016/j.fsigen.2014.02.006
M3 - Article
C2 - 24631692
AN - SCOPUS:84896532015
VL - 11
SP - 7
EP - 12
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
SN - 1872-4973
IS - 1
ER -