miR-214 regulates lactoferrin expression and pro-apoptotic function in mammary epithelial cells

Yalin Liao, Xiaogu Du, Bo Lönnerdal

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Lactoferrin (Lf) is an abundantly expressed protein in human milk. Lactoferrin exhibits several important biological functions, and its expression is regulated by multiple environmental factors. Cellular endogenous factors, however, have not been extensively studied with regard to lactoferrin gene expression. In this study, we showed that lactoferrin gene expression and function are directly targeted by miR-214 in HC11 and MCF7 cells. In the lactoferrin mRNA 39 untranslated region (UTR) of human, mouse, rat, pig, bovine, camel, and goat species, there is a conserved region that perfectly matches the seed region of miR-214. Transfection of miR-214 mimic in HEK293 cells dose-dependently inhibited the activity of pGL3-control vector containing lactoferrin mRNA 3′UTR downstream of the luciferase gene. In HC11 cells, miR-214 overexpression inhibited the induction of lactoferrin expression by β-estradiol (E2) and dexamethasone-prolactin-insulin (DPI). Furthermore, in MCF7 cells, overexpression of miR-214 markedly decreased lactoferrin expression (P < 0.05), and inhibition of endogenous miR-214 expression increased lactoferrin expression and cellular apoptotic activities (P < 0.05). In summary, our data showed that miR-214 is directly involved in lactoferrin expression and lactoferrin mediated cancer susceptibility (proapoptotic activities) in mammary epithelial cells.

Original languageEnglish (US)
Pages (from-to)1552-1556
Number of pages5
JournalJournal of Nutrition
Volume140
Issue number9
DOIs
StatePublished - Sep 2010

Fingerprint

Lactoferrin
Breast
Epithelial Cells
MCF-7 Cells
Untranslated Regions
Gene Expression
Camelus
Messenger RNA
HEK293 Cells
Human Milk
Luciferases
Goats
Prolactin
Dexamethasone
Transfection
Estradiol
Seeds
Swine
Insulin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

miR-214 regulates lactoferrin expression and pro-apoptotic function in mammary epithelial cells. / Liao, Yalin; Du, Xiaogu; Lönnerdal, Bo.

In: Journal of Nutrition, Vol. 140, No. 9, 09.2010, p. 1552-1556.

Research output: Contribution to journalArticle

Liao, Yalin ; Du, Xiaogu ; Lönnerdal, Bo. / miR-214 regulates lactoferrin expression and pro-apoptotic function in mammary epithelial cells. In: Journal of Nutrition. 2010 ; Vol. 140, No. 9. pp. 1552-1556.
@article{4a909213b06542089f26d0e611f7bed1,
title = "miR-214 regulates lactoferrin expression and pro-apoptotic function in mammary epithelial cells",
abstract = "Lactoferrin (Lf) is an abundantly expressed protein in human milk. Lactoferrin exhibits several important biological functions, and its expression is regulated by multiple environmental factors. Cellular endogenous factors, however, have not been extensively studied with regard to lactoferrin gene expression. In this study, we showed that lactoferrin gene expression and function are directly targeted by miR-214 in HC11 and MCF7 cells. In the lactoferrin mRNA 39 untranslated region (UTR) of human, mouse, rat, pig, bovine, camel, and goat species, there is a conserved region that perfectly matches the seed region of miR-214. Transfection of miR-214 mimic in HEK293 cells dose-dependently inhibited the activity of pGL3-control vector containing lactoferrin mRNA 3′UTR downstream of the luciferase gene. In HC11 cells, miR-214 overexpression inhibited the induction of lactoferrin expression by β-estradiol (E2) and dexamethasone-prolactin-insulin (DPI). Furthermore, in MCF7 cells, overexpression of miR-214 markedly decreased lactoferrin expression (P < 0.05), and inhibition of endogenous miR-214 expression increased lactoferrin expression and cellular apoptotic activities (P < 0.05). In summary, our data showed that miR-214 is directly involved in lactoferrin expression and lactoferrin mediated cancer susceptibility (proapoptotic activities) in mammary epithelial cells.",
author = "Yalin Liao and Xiaogu Du and Bo L{\"o}nnerdal",
year = "2010",
month = "9",
doi = "10.3945/jn.110.124289",
language = "English (US)",
volume = "140",
pages = "1552--1556",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "9",

}

TY - JOUR

T1 - miR-214 regulates lactoferrin expression and pro-apoptotic function in mammary epithelial cells

AU - Liao, Yalin

AU - Du, Xiaogu

AU - Lönnerdal, Bo

PY - 2010/9

Y1 - 2010/9

N2 - Lactoferrin (Lf) is an abundantly expressed protein in human milk. Lactoferrin exhibits several important biological functions, and its expression is regulated by multiple environmental factors. Cellular endogenous factors, however, have not been extensively studied with regard to lactoferrin gene expression. In this study, we showed that lactoferrin gene expression and function are directly targeted by miR-214 in HC11 and MCF7 cells. In the lactoferrin mRNA 39 untranslated region (UTR) of human, mouse, rat, pig, bovine, camel, and goat species, there is a conserved region that perfectly matches the seed region of miR-214. Transfection of miR-214 mimic in HEK293 cells dose-dependently inhibited the activity of pGL3-control vector containing lactoferrin mRNA 3′UTR downstream of the luciferase gene. In HC11 cells, miR-214 overexpression inhibited the induction of lactoferrin expression by β-estradiol (E2) and dexamethasone-prolactin-insulin (DPI). Furthermore, in MCF7 cells, overexpression of miR-214 markedly decreased lactoferrin expression (P < 0.05), and inhibition of endogenous miR-214 expression increased lactoferrin expression and cellular apoptotic activities (P < 0.05). In summary, our data showed that miR-214 is directly involved in lactoferrin expression and lactoferrin mediated cancer susceptibility (proapoptotic activities) in mammary epithelial cells.

AB - Lactoferrin (Lf) is an abundantly expressed protein in human milk. Lactoferrin exhibits several important biological functions, and its expression is regulated by multiple environmental factors. Cellular endogenous factors, however, have not been extensively studied with regard to lactoferrin gene expression. In this study, we showed that lactoferrin gene expression and function are directly targeted by miR-214 in HC11 and MCF7 cells. In the lactoferrin mRNA 39 untranslated region (UTR) of human, mouse, rat, pig, bovine, camel, and goat species, there is a conserved region that perfectly matches the seed region of miR-214. Transfection of miR-214 mimic in HEK293 cells dose-dependently inhibited the activity of pGL3-control vector containing lactoferrin mRNA 3′UTR downstream of the luciferase gene. In HC11 cells, miR-214 overexpression inhibited the induction of lactoferrin expression by β-estradiol (E2) and dexamethasone-prolactin-insulin (DPI). Furthermore, in MCF7 cells, overexpression of miR-214 markedly decreased lactoferrin expression (P < 0.05), and inhibition of endogenous miR-214 expression increased lactoferrin expression and cellular apoptotic activities (P < 0.05). In summary, our data showed that miR-214 is directly involved in lactoferrin expression and lactoferrin mediated cancer susceptibility (proapoptotic activities) in mammary epithelial cells.

UR - http://www.scopus.com/inward/record.url?scp=77956595725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956595725&partnerID=8YFLogxK

U2 - 10.3945/jn.110.124289

DO - 10.3945/jn.110.124289

M3 - Article

C2 - 20610637

AN - SCOPUS:77956595725

VL - 140

SP - 1552

EP - 1556

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 9

ER -