MiR-146a and miR-155 delineate a microRNA fingerprint associated with toxoplasma persistence in the host brain

Dominique Cannella, Marie Pierre Brenier-Pinchart, Laurence Braun, Jason M. vanRooyen, Alexandre Bougdour, Olivier Bastien, Michael S. Behnke, Rose Laurence Curt, Aurélie Curt, Jeroen Saeij, L. David Sibley, Hervé Pelloux, Mohamed Ali Hakimi

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

microRNAs were recently found to be regulators of the host response to infection by apicomplexan parasites. In this study, we identified two immunomodulatory microRNAs, miR-146a and miR-155, that werecoinduced in the brains of mice challenged with Toxoplasma in a strain-specific manner. These microRNAs define a characteristic fingerprint for infection by type II strains, which are the most prevalent cause of human toxoplasmosis in Europe and North America. Using forward genetics, we showed that strain-specific differences in miR-146a modulation were in part mediated by the rhoptry kinase, ROP16. Remarkably, we found that miR-146a deficiency led to better control of parasite burden in the gut and most likely of early parasite dissemination in the brain tissue, resulting in the long-term survival of mice.

Original languageEnglish (US)
Pages (from-to)928-937
Number of pages10
JournalCell Reports
Volume6
Issue number5
DOIs
StatePublished - 2014
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cannella, D., Brenier-Pinchart, M. P., Braun, L., vanRooyen, J. M., Bougdour, A., Bastien, O., Behnke, M. S., Curt, R. L., Curt, A., Saeij, J., Sibley, L. D., Pelloux, H., & Hakimi, M. A. (2014). MiR-146a and miR-155 delineate a microRNA fingerprint associated with toxoplasma persistence in the host brain. Cell Reports, 6(5), 928-937. https://doi.org/10.1016/j.celrep.2014.02.002