Minipreps of plasmid DNA.

J. Engebrecht, R. Brent, M. A. Kaderbhai

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Although there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5-ml tubes or 96-well microtiter dishes, the boiling method, and a lithium-based procedure. A support protocol provides information on storing plasmid DNA.

Original languageEnglish (US)
JournalCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
VolumeChapter 1
StatePublished - May 2001
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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