Minipreps of plasmid DNA.

J. Engebrecht; R. Brent; M. A. Kaderbhai

Minipreps of plasmid DNA.

J. Engebrecht, R. Brent, M. A. Kaderbhai

Research output: Contribution to journal › Article › peer-review

Abstract

Although there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5-ml tubes or 96-well microtiter dishes, the boiling method, and a lithium-based procedure. A support protocol provides information on storing plasmid DNA.

Original language	English (US)
Journal	Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
Volume	Chapter 1
State	Published - May 2001
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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title = "Minipreps of plasmid DNA.",

abstract = "Although there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5-ml tubes or 96-well microtiter dishes, the boiling method, and a lithium-based procedure. A support protocol provides information on storing plasmid DNA.",

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