Mincle and human B cell function

Kazuhito Kawata, Petr Illarionov, Guo Xiang Yang, Thomas P. Kenny, Weici Zhang, Masanobu Tsuda, Yugo Ando, Patrick S Leung, Aftab A. Ansari, M. Eric Gershwin

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

C-type lectin receptors are pattern recognition receptors that are critical for autoimmunity and the immune response. Mincle is a C-type lectin receptor expressed by a variety of antigen presenting cells including macrophages, neutrophils, dendritic cells and B cells; a variety of stimuli including stress are known to induce the expression of Mincle. Mincle is an FcRγ-associated activation receptor that senses damaged cells and upon ligation induces activated macrophages to produce inflammatory cytokines. Recently, while several studies have reported that Mincle plays an important role in macrophage responses to fungal infection its function on B cells remains to be defined. In efforts to elucidate the function of Mincle expressed by B cells, we studied the expression of Mincle on subsets of B cells and analyzed cytokines and synthesized immunoglobulin upon ligation of Mincle. The expression of Mincle on CD27-CD19+ naïve B cells is significantly higher than CD27 + CD19+ memory B cells. The stimulation of TLR9 ligand induced Mincle expression on B cells. Furthermore, co-stimulation of TLR9 and Mincle ligand reduced IgG and IgA production from B cells without a significant change in the inflammatory cytokines TNF-α, IL-6, IL-8 and IL-10. Our data identifies Mincle as a potentially critical player in human B cell responses.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalJournal of Autoimmunity
Volume39
Issue number4
DOIs
StatePublished - Dec 2012

Keywords

  • C-type lectin
  • Mincle
  • Toll-like receptor

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Fingerprint Dive into the research topics of 'Mincle and human B cell function'. Together they form a unique fingerprint.

  • Cite this

    Kawata, K., Illarionov, P., Yang, G. X., Kenny, T. P., Zhang, W., Tsuda, M., Ando, Y., Leung, P. S., Ansari, A. A., & Gershwin, M. E. (2012). Mincle and human B cell function. Journal of Autoimmunity, 39(4), 315-322. https://doi.org/10.1016/j.jaut.2012.04.004